Literature summary for 2.1.1.142 extracted from

Zhou, W.; Song, Z.; Kanagasabai, R.; Liu, J.; Jayasimha, P.; Sinha, A.; Veeramachanemi, P.; Miller, M.B.; Nes, W.D.
Mechanism-based enzyme inactivators of phytosterol biosynthesis (2004), Molecules, 9, 185-203.

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Inhibitors

Inhibitors	Comment	Organism	Structure
24(28)-methylenencycloartanol	-	Glycine max
26,27-dehydrocycloartenol	-	Glycine max
cycloarta-24,26-dienol	-	Glycine max
additional information	neither ergosterol or cholesterol inhibit activity	Glycine max
sitosterol	-	Glycine max

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.01	-	26,27-dehydrocycloartenol	-	Glycine max
0.022	-	24(28)-methylenelophenol	-	Glycine max
0.03	-	Cycloartenol	-	Glycine max

Organism

Organism	UniProt	Comment	Textmining
Glycine max	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + 24(28)-methylenelophenol	-	Glycine max	S-adenosyl-L-homocysteine + ?	-	?
S-adenosyl-L-methionine + 26,27-dehydrocycloartenol	-	Glycine max	S-adenosyl-L-homocysteine + ?	-	?
S-adenosyl-L-methionine + cycloartenol	the first C1-transfer is 24(28) methylenecycloartenol, SN2-type and non-stop, ternary complex mechanism. The second C1-transfer is a mixture of 24-ethyl olefins, SN2-type and step-wise. Ordered pathway with S-adenosyl-L-methionine binding first	Glycine max	S-adenosyl-L-homocysteine + ?	-	?

Synonyms

Synonyms	Comment	Organism
SMT1	-	Glycine max
sterol methyl transferase	-	Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00033	-	26,27-dehydrocycloartenol	-	Glycine max
0.001	-	24(28)-methylenelophenol	-	Glycine max
0.01	-	Cycloartenol	-	Glycine max

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.017	-	cycloarta-24,26-dienol	-	Glycine max
0.042	-	26,27-dehydrocycloartenol	-	Glycine max
0.1	-	sitosterol	-	Glycine max
0.171	-	24(28)-methylenencycloartanol	-	Glycine max

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Release 2023.1 (January 2023)