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Literature summary for 2.1.1.142 extracted from
- Structural requirements for transformation of substrates by the (S)-adenosyl-L-methionine:delta 24(25)-sterol methyl transferase (1991), J. Biol. Chem., 266, 15202-15212.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no cofactor requirement | Helianthus annuus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.028 | - |
lanosterol | - |
Helianthus annuus |  |
| 0.032 | - |
Cycloartenol | - |
Helianthus annuus | |
| 0.033 | - |
14alpha-methylzymosterol | - |
Helianthus annuus | |
| 0.036 | - |
31-norcycloartenol | - |
Helianthus annuus | |
| 0.043 | - |
24,25-Dehydropollinastanol | - |
Helianthus annuus | |
| 0.063 | - |
Zymosterol | - |
Helianthus annuus | |
| 0.067 | - |
desmosterol | lanosta-7,24-dienol | Helianthus annuus | |
| 0.125 | - |
Cholesta-5,7,24-trienol | - |
Helianthus annuus | |
| 0.13 | - |
Agnosterol | - |
Helianthus annuus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Helianthus annuus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no metal ion required | Helianthus annuus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| zymosterol + S-adenosyl-L-methionine | Helianthus annuus | - |
fecosterol + S-adenosyl-L-homocysteine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helianthus annuus | - |
sunflower | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol | mechanism | Helianthus annuus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Helianthus annuus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 14alpha-methylzymosterol + S-adenosyl-L-methionine | 14alpha-methylzymosterol is 14alpha-methylcholesta-8,24-dien-3beta-ol | Helianthus annuus | 14alpha-methyl-24-methylenezymosterol + S-adenosyl-L-homocysteine | - |
? | |
| 24,25-dehydropollinastanol + S-adenosyl-L-methionine | 24,25-dehydropollinastanol is 14alpha-methyl-9beta,19-cyclo-5alpha-cholest-24-en-3beta-ol | Helianthus annuus | ? | - |
? | |
| 31-norcycloartenol + S-adenosyl-L-methionine | 31-norcycloartenol is 4,14alpha-dimethyl-9beta,19-cyclo-5alpha-cholest-24-en-3beta-ol | Helianthus annuus | 24-methylene-31-norcycloartenol + S-adenosyl-L-homocysteine | - |
? | |
| agnosterol + S-adenosyl-L-methionine | agnosterol is 4,4,14alpha-trimethyl-5alpha-cholesta-7(8),9(11),24-trien-3beta-ol | Helianthus annuus | 24-methyleneagnosterol + S-adenosyl-L-homocysteine | - |
? | |
| cholesta-5,7,24-trienol + S-adenosyl-L-methionine | - |
Helianthus annuus | ? | - |
? | |
| cycloartenol + S-adenosyl-L-methionine | cycloartenol is 4,4,14alpha-trimethyl-9beta,19-cyclo-5alpha-cholest-24-en-3beta-ol | Helianthus annuus | 24-methylenecycloartanol + S-adenosyl-L-homocysteine | - |
? | |
| cycloartenol 3-ketone + S-adenosyl-L-methionine | cycloartenol 3-ketone is 4,4,14alpha-trimethyl-9beta,19-cyclo-5alpha-cholest-24-en-3-one | Helianthus annuus | ? | - |
? | |
| desmosterol + S-adenosyl-L-methionine | desmosterol is 5alpha-cholesta-5,24-dien-3beta-ol | Helianthus annuus | 24-methylenedesmosterol + S-adenosyl-L-homocysteine | - |
? | |
| lanost-24-enol + S-adenosyl-L-methionine | lanost-24-enol is 4,4,14alpha-trimethyl-5alpha-cholesta-9(11),24-dien-3beta-ol | Helianthus annuus | 24-methylene-lanost-24-erol + S-adenosyl-L-homocysteine | - |
? | |
| lanosta-7,24-dienol + S-adenosyl-L-methionine | lanosta-7,24-dienol is 4,4,14alpha-trimethyl-5alpha-cholesta-7(8),24-dien-3beta-ol | Helianthus annuus | 24-methylene-lanost-7,24-dienol + S-adenosyl-L-homocysteine | - |
? | |
| lanosterol + S-adenosyl-L-methionine | lanosterol is 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3-ol | Helianthus annuus | 24-methylene-24,25-dihydrolanosterol + S-adenosyl-L-homocysteine | - |
? | |
| zymosterol + S-adenosyl-L-methionine | - |
Helianthus annuus | fecosterol + S-adenosyl-L-homocysteine | - |
? | |
| zymosterol + S-adenosyl-L-methionine | zymosterol is 5alpha-cholesta-8,24-dien-3beta-ol | Helianthus annuus | fecosterol + S-adenosyl-L-homocysteine | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Helianthus annuus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | slightly active at pH 5.5, no activity at pH 10 | Helianthus annuus |
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