Protein Variants | Comment | Organism |
---|---|---|
N361S | the mutation leads to higher specific activity with benzoate than with jasmonate | Populus trichocarpa |
S153Y | the mutation leads to strongly reduced activity with benzoate or jasmonate | Populus trichocarpa |
S153Y/N361S | the mutation leads to strongly reduced activity with benzoate or jasmonate | Populus trichocarpa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM Ca2+ shows a mild inhibition effect on enzyme activity (about 80% residual activity) | Populus trichocarpa | |
Cu2+ | complete inhibition at 5 mM | Populus trichocarpa | |
Fe2+ | complete inhibition at 5 mM | Populus trichocarpa | |
Fe3+ | complete inhibition at 5 mM | Populus trichocarpa | |
Mg2+ | shows a mild inhibition effect on enzyme activity (about 85% residual activity) | Populus trichocarpa | |
Mn2+ | 5 mM Mn2+ decreases the enzyme activity by 40% | Populus trichocarpa | |
Zn2+ | complete inhibition at 5 mM | Populus trichocarpa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0631 | - |
benzoate | mutant enzyme N361S, at pH 6.5 and 30°C | Populus trichocarpa | |
0.175 | - |
jasmonate | wild type enzyme, at pH 6.5 and 30°C | Populus trichocarpa | |
0.31 | - |
jasmonate | mutant enzyme N361S, at pH 6.5 and 30°C | Populus trichocarpa | |
0.341 | - |
benzoate | wild type enzyme, at pH 6.5 and 30°C | Populus trichocarpa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | significantly stimulates enzyme activity (to about 250%) | Populus trichocarpa | |
Na+ | has a mild stimulation on enzyme activity (to about 160%) | Populus trichocarpa | |
NH4+ | has a mild stimulation on enzyme activity (to about 165%) | Populus trichocarpa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + jasmonate | Populus trichocarpa | - |
S-adenosyl-L-homocysteine + methyl jasmonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus trichocarpa | B9H4E1 | black cottonwood | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Populus trichocarpa | - |
seedling | - |
Populus trichocarpa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with salicylic acid, indole-3-acetic acid, and gibberellic acid | Populus trichocarpa | ? | - |
? | |
S-adenosyl-L-methionine + benzoate | 22% activity compared to jasmonate | Populus trichocarpa | S-adenosyl-L-homocysteine + methyl benzoate | - |
? | |
S-adenosyl-L-methionine + jasmonate | - |
Populus trichocarpa | S-adenosyl-L-homocysteine + methyl jasmonate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
jasmonic acid methyltransferase | - |
Populus trichocarpa |
JMT1 | - |
Populus trichocarpa |
S-adenosyl-L-methionine:jasmonic acid carboxyl methyltransferase | - |
Populus trichocarpa |
SABATH3 | formerly | Populus trichocarpa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 60 | the enzyme is 100% stable for 30 min at 4°C. It loses about 10%, 20% and 40% of its maximal activity when incubated at 37°C, 42°C and 50°C, respectively for 30 min. When incubated at 60°C for 30 min, the enzyme loses all of its activity | Populus trichocarpa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
benzoate | wild type enzyme, at pH 6.5 and 30°C | Populus trichocarpa | |
0.013 | - |
jasmonate | wild type enzyme, at pH 6.5 and 30°C | Populus trichocarpa | |
0.015 | - |
jasmonate | mutant enzyme N361S, at pH 6.5 and 30°C | Populus trichocarpa | |
0.028 | - |
benzoate | mutant enzyme N361S, at pH 6.5 and 30°C | Populus trichocarpa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Populus trichocarpa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | at pH 6.0, pH 7.0, pH 8.0 and pH 8.5, the enzyme displays about 80%, 50%, 20% and 5% of its maximal activity, respectively | Populus trichocarpa |
Organism | Comment | Expression |
---|---|---|
Populus trichocarpa | the enzyme expression is induced by methyl jasmonate and salicylic acid as well as alamethicin | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme plays a role in plant defense against biotic stresses | Populus trichocarpa |