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Literature summary for 2.1.1.14 extracted from

  • Mordukhova, E.A.; Pan, J.G.
    Evolved cobalamin-independent methionine synthase (MetE) improves the acetate and thermal tolerance of Escherichia coli (2013), Appl. Environ. Microbiol., 79, 7905-7915.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
V39A/R46C/T106I/K713E mutant confers accelerated growth in the Escherichia coli K-12 WE strain in the presence of acetate. Strains harboring acetate-tolerant MetE mutants are less inhibited by homocysteine in L-isoleucine-enriched medium. The acetate-tolerant MetE mutants stimulate the growth of the host strain at elevated temperatures of 44 and 45°C. The mutant MetE enzymes display a reduced melting temperature but an enhanced in vivo stability Escherichia coli
V39A/R46C/T106I/K713E/C645A C645A mutation additionally improves acetate tolerance. Strains harboring acetate-tolerant MetE mutants are less inhibited by homocysteine in L-isoleucine-enriched medium. The acetate-tolerant MetE mutants stimulate the growth of the host strain at elevated temperatures of 44 and 45°C. The mutant MetE enzymes display a reduced melting temperature but an enhanced in vivo stability Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P25665
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-

Synonyms

Synonyms Comment Organism
MetE
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48.5
-
 melting temperature, mutant V39A/R46C/T106I/K713E/C645A Escherichia coli
51.3
-
 melting temperature, mutant V39A/R46C/T106I/K713E Escherichia coli
54.7
-
 melting temperature, wild-type Escherichia coli