Literature summary for 2.1.1.14 extracted from

Fu, T.M.; Almqvist, J.; Liang, Y.H.; Li, L.; Huang, Y.; Su, X.D.
Crystal structures of cobalamin-independent methionine synthase (MetE) from Streptococcus mutans: a dynamic zinc-inversion model (2011), J. Mol. Biol., 412, 688-697.

Search for more literature for 2.1.1.14

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)	Streptococcus mutans

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapor diffusion method	Streptococcus mutans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	-	Streptococcus mutans

Organism

Organism	UniProt	Comment	Textmining
Streptococcus mutans	Q8CWX6	-	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal ion affinity chromatography (Ni2+), gel fitration	Streptococcus mutans

Subunits

Subunits	Comment	Organism
monomer	gel filtration	Streptococcus mutans

Synonyms

Synonyms	Comment	Organism
cobalamin-independent methionine synthase (MetE)	-	Streptococcus mutans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	0.155 micromol/min/mg, at pH 6.0: 0.152 micromol/min/mg, at pH 7.0: 0.148 micromol/min/mg, at pH 4.6: 0.0096 micromol/min/mg, at pH 9.0: 0.119 micromol/min/mg	Streptococcus mutans

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.6	9	at pH 4.6: 0.0096 micromol/min/mg, at pH 6.5: 0.155 micromol/min/mg, at pH 9.0: 0.119 micromol/min/mg	Streptococcus mutans

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Release 2023.1 (January 2023)