Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methionine | - |
Daucus carota | |
methionine | - |
Hordeum vulgare | |
methionine | - |
Phaseolus sp. | |
methionine | - |
Pisum sativum | |
methionine | - |
Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000026 | - |
5-methyltetrahydropteroylglutamate | - |
Pisum sativum | |
0.0022 | - |
S-methylmethionine | - |
Pisum sativum | |
0.004 | - |
S-adenosylmethionine | - |
Pisum sativum | |
0.041 | - |
homocysteine | cosubstrate S-methyl-L-methionine | Phaseolus sp. | |
0.055 | - |
S-methylmethionine | - |
Phaseolus sp. | |
0.6 | - |
homocysteine | cosubstrate 5-methyltetrahydropteroylglutamate | Pisum sativum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Hordeum vulgare | 9507 | - |
chloroplast | - |
Spinacia oleracea | 9507 | - |
chloroplast | - |
Pisum sativum | 9507 | - |
chloroplast | - |
Daucus carota | 9507 | - |
chloroplast | - |
Phaseolus sp. | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulates | Hordeum vulgare | |
Mg2+ | stimulates | Spinacia oleracea | |
Mg2+ | stimulates | Pisum sativum | |
Mg2+ | stimulates | Daucus carota | |
Mg2+ | stimulates | Phaseolus sp. | |
phosphate | - |
Hordeum vulgare | |
phosphate | - |
Spinacia oleracea | |
phosphate | - |
Pisum sativum | |
phosphate | - |
Daucus carota | |
phosphate | - |
Phaseolus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Hordeum vulgare | final step in methionine synthesis | ? | - |
? | |
additional information | Spinacia oleracea | final step in methionine synthesis | ? | - |
? | |
additional information | Pisum sativum | final step in methionine synthesis | ? | - |
? | |
additional information | Daucus carota | final step in methionine synthesis | ? | - |
? | |
additional information | Phaseolus sp. | final step in methionine synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daucus carota | - |
carrot | - |
Hordeum vulgare | - |
barley | - |
Phaseolus sp. | - |
bean | - |
Pisum sativum | - |
pea, 2 enzyme forms: a 5-methyltetrahydropteroylglutamate dependent enzyme and a S-methylmethionine dependent enzyme | - |
Spinacia oleracea | - |
spinach | - |
Purification (Comment) | Organism |
---|---|
- |
Phaseolus sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine | final step in methionine synthesis | Hordeum vulgare | |
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine | final step in methionine synthesis | Spinacia oleracea | |
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine | final step in methionine synthesis | Pisum sativum | |
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine | final step in methionine synthesis | Daucus carota | |
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine | final step in methionine synthesis | Phaseolus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Hordeum vulgare | - |
root | - |
Spinacia oleracea | - |
root | - |
Pisum sativum | - |
root | - |
Daucus carota | - |
root | - |
Phaseolus sp. | - |
seedling | - |
Hordeum vulgare | - |
seedling | - |
Spinacia oleracea | - |
seedling | - |
Pisum sativum | - |
seedling | - |
Daucus carota | - |
seedling | - |
Phaseolus sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine | - |
Hordeum vulgare | tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine | - |
? | |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine | - |
Spinacia oleracea | tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine | - |
? | |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine | - |
Pisum sativum | tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine | - |
? | |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine | - |
Daucus carota | tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine | - |
? | |
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine | - |
Phaseolus sp. | tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine | - |
? | |
additional information | enzymes of higher plants can use the monoglutamate | Hordeum vulgare | ? | - |
? | |
additional information | enzymes of higher plants can use the monoglutamate | Spinacia oleracea | ? | - |
? | |
additional information | enzymes of higher plants can use the monoglutamate | Pisum sativum | ? | - |
? | |
additional information | enzymes of higher plants can use the monoglutamate | Daucus carota | ? | - |
? | |
additional information | enzymes of higher plants can use the monoglutamate | Phaseolus sp. | ? | - |
? | |
additional information | most active with the polyglutamate forms of N5-methyltetrahydropteroylglutamate | Hordeum vulgare | ? | - |
? | |
additional information | most active with the polyglutamate forms of N5-methyltetrahydropteroylglutamate | Spinacia oleracea | ? | - |
? | |
additional information | most active with the polyglutamate forms of N5-methyltetrahydropteroylglutamate | Pisum sativum | ? | - |
? | |
additional information | most active with the polyglutamate forms of N5-methyltetrahydropteroylglutamate | Daucus carota | ? | - |
? | |
additional information | most active with the polyglutamate forms of N5-methyltetrahydropteroylglutamate | Phaseolus sp. | ? | - |
? | |
additional information | final step in methionine synthesis | Hordeum vulgare | ? | - |
? | |
additional information | final step in methionine synthesis | Spinacia oleracea | ? | - |
? | |
additional information | final step in methionine synthesis | Pisum sativum | ? | - |
? | |
additional information | final step in methionine synthesis | Daucus carota | ? | - |
? | |
additional information | final step in methionine synthesis | Phaseolus sp. | ? | - |
? | |
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine | - |
Pisum sativum | tetrahydropteroylmonoglutamate + L-methionine | - |
? | |
S-adenosylmethionine + homocysteine | - |
Pisum sativum | methionine + S-adenosylhomocysteine | - |
? | |
S-methylmethionine + homocysteine | - |
Pisum sativum | methionine | - |
? | |
S-methylmethionine + L-homocysteine | - |
Phaseolus sp. | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
5-methyltetrahydropteroylglutamate | Pisum sativum |
7.3 | - |
L-methionine | Pisum sativum |
7.9 | - |
L-methionine | Phaseolus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | vitamin-B12 independent enzyme | Hordeum vulgare | |
additional information | vitamin-B12 independent enzyme | Spinacia oleracea | |
additional information | vitamin-B12 independent enzyme | Pisum sativum | |
additional information | vitamin-B12 independent enzyme | Daucus carota | |
additional information | vitamin-B12 independent enzyme | Phaseolus sp. | |
additional information | no requirement for a reducing system | Hordeum vulgare | |
additional information | no requirement for a reducing system | Spinacia oleracea | |
additional information | no requirement for a reducing system | Pisum sativum | |
additional information | no requirement for a reducing system | Daucus carota | |
additional information | no requirement for a reducing system | Phaseolus sp. | |
additional information | no requirement for S-adenosylmethionine | Hordeum vulgare | |
additional information | no requirement for S-adenosylmethionine | Spinacia oleracea | |
additional information | no requirement for S-adenosylmethionine | Pisum sativum | |
additional information | no requirement for S-adenosylmethionine | Daucus carota | |
additional information | no requirement for S-adenosylmethionine | Phaseolus sp. |