Any feedback?
Literature summary for 2.1.1.137 extracted from
- Identification of an S-adenosylmethionine (SAM) dependent arsenic methyltransferase in Danio rerio (2012), Toxicol. Appl. Pharmacol., 262, 185-193.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Danio rerio |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C165S | inactive | Danio rerio |
| C210S | inactive | Danio rerio |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenite | the enzyme activity is completely inhibited by elevated concentrations of the substrate arsenite (0.2 mM) | Danio rerio |  |
| Selenite | addition of selenite at 1:10 molar ratio with arsenite in the reaction mixture completely abolishes the arsenic methylation | Danio rerio | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
x * 80000, SDS-PAGE | Danio rerio |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Danio rerio | - |
line ZF-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Danio rerio |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Danio rerio | - |
| eye | - |
Danio rerio | - |
| gill | - |
Danio rerio | - |
| heart | - |
Danio rerio | - |
| intestine | prominent expression in intestine | Danio rerio | - |
| kidney | - |
Danio rerio | - |
| liver | most prominent expression in liver | Danio rerio | - |
| additional information | the enzyme is universally expressed in zebrafish tissues | Danio rerio | - |
| muscle | - |
Danio rerio | - |
| skin | - |
Danio rerio | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + arsenite | the enzyme methylates arsenite to dimethylarsinic acid as an end product and produces monomethylarsonous acid and monomethylarsonic acid as intermediates | Danio rerio | S-adenosyl-L-homocysteine + monomethylarsonous acid + monomethylarsonic acid + dimethylarsinic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80000, SDS-PAGE | Danio rerio |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AS3MT | - |
Danio rerio |
| S-adenosylmethionine dependent arsenic methyltransferase | - |
Danio rerio |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
