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Literature summary for 2.1.1.137 extracted from

  • Marapakala, K.; Qin, J.; Rosen, B.P.
    Identification of catalytic residues in the As(III) S-adenosylmethionine methyltransferase (2012), Biochemistry, 51, 944-951.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.137

Activating Compound

Activating Compound Comment Organism Structure
glutathione As(III) SAM methyltransferase conducts three successive rounds of oxidative methylation, with the glutathione conjugates serving as metalloid donors to the enzyme Cyanidioschyzon sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Cyanidioschyzon sp.

Protein Variants

Protein Variants Comment Organism
C174A the mutation leads to loss of As(III) methylation Cyanidioschyzon sp.
C224A the mutation leads to loss of As(III) methylation Cyanidioschyzon sp.
C72A the mutation leads to loss of As(III) methylation, but still shows trivalent methylarsenate methylation Cyanidioschyzon sp.
Y70W the mutant exhibits metalloid binding Cyanidioschyzon sp.

Organism

Organism UniProt Comment Textmining
Cyanidioschyzon sp.
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-
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Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Cyanidioschyzon sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + arsenite the first product, methylarsonate, undergoes a second round of methylation faster than it dissociates from the enzyme. The second product, dimethylarsinate, dissociates faster than it undergoes the third round of methylation to the third and final product, trimethylarsine Cyanidioschyzon sp. S-adenosyl-L-homocysteine + methylarsonate + dimethylarsinate + trimethylarsine
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Synonyms

Synonyms Comment Organism
ArsM
-
 Cyanidioschyzon sp.
As(III) S-adenosylmethionine methyltransferase
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 Cyanidioschyzon sp.
As(III) SAM methyltransferase
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 Cyanidioschyzon sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70
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 Cyanidioschyzon sp.