Cloned (Comment) | Organism |
---|---|
expressed in Sf9 insect cells | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cobalamin affinity chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.94 | - |
after 279fold purification at 37°C | Homo sapiens |
2.53 | - |
crude cell extract at 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine | - |
Homo sapiens | tetrahydropteroylmonoglutamate + L-methionine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MS | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
the apoenzyme preincubated in the absence of methyl synthase reductase almost completely loses the ability to form holoenzyme after the subsequent addition of methylcobalamin and incubation at 37°C for 10 min | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
vitamin B12 | required for activity | Homo sapiens |