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Literature summary for 2.1.1.13 extracted from

  • Yamada, K.; Gravel, R.A.; Toraya, T.; Matthews, R.G.
    Human methionine synthase reductase is a molecular chaperone for human methionine synthase (2006), Proc. Natl. Acad. Sci. USA, 103, 9476-9481.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cobalamin affinity chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.94
-
after 279fold purification at 37°C Homo sapiens
2.53
-
crude cell extract at 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
Homo sapiens tetrahydropteroylmonoglutamate + L-methionine
-
?

Synonyms

Synonyms Comment Organism
MS
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
the apoenzyme preincubated in the absence of methyl synthase reductase almost completely loses the ability to form holoenzyme after the subsequent addition of methylcobalamin and incubation at 37°C for 10 min Homo sapiens

Cofactor

Cofactor Comment Organism Structure
vitamin B12 required for activity Homo sapiens