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Literature summary for 2.1.1.13 extracted from

  • Banks, E.C.; Doughty, S.W.; Toms, S.M.; Wheelhouse, R.T.; Nicolaou, A.
    Inhibition of cobalamin-dependent methionine synthase by substituted benzo-fused heterocycles (2007), FEBS J., 274, 287-299.
    View publication on PubMed
Search for more literature for 2.1.1.13

Inhibitors

Inhibitors Comment Organism Structure
1-methyl-5-amino-benzimidazole IC50: more than 0.15 mM Rattus norvegicus
1-methyl-5-nitro-7-methoxybenzimidazole IC50: 0.15 mM Rattus norvegicus
1-methyl-5-nitro-benzimidazole IC50: more than 0.15 mM Rattus norvegicus
1-methylbenzimidazole IC50: more than 0.15 mM Rattus norvegicus
2,1,3-benzothiadiazole IC50: more than 0.15 mM Rattus norvegicus
2-nitro-1H-benzimidazole IC50: 0.12 mM Rattus norvegicus
4-amino-2,1,3-benzothiadiazole IC50: more than 0.15 mM Rattus norvegicus
4-nitro-2,1,3-benzothiadiazole IC50: 0.08 mM Rattus norvegicus
5-amino-7-methoxy-N1-methylbenzimidazole IC50: 0.095 mM Rattus norvegicus
5-amino-7-methoxybenzimidazole IC50: more than 0.15 mM Rattus norvegicus
5-aminobenzimidazole IC50: more than 0.15 mM Rattus norvegicus
5-methoxybenzimidazole IC50: more than 0.15 mM Rattus norvegicus
5-nitro-7-methoxybenzimidazole IC50: 0.1 mM Rattus norvegicus
benzimidazole IC50: more than 0.15 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 L-homocysteine in 50 mM phosphate buffer, pH 7.4, at 37°C Rattus norvegicus
0.025
-
 N5-methyltetrahydropteroylmonoglutamate in 50 mM phosphate buffer, pH 7.4, at 37°C Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE cellulose batch chromatography and Q-sepharose column chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Rattus norvegicus tetrahydropteroylmonoglutamate + L-methionine
-
 ?

Synonyms

Synonyms Comment Organism
5-methyltetrahydrofolate-homocysteine transmethylase
-
 Rattus norvegicus
MetS
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
vitamin B12 dependent Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.017
-
 4-nitro-2,1,3-benzothiadiazole in 50 mM phosphate buffer, pH 7.4, at 37°C Rattus norvegicus
0.026
-
 2-nitro-1H-benzimidazole in 50 mM phosphate buffer, pH 7.4, at 37°C Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.08
-
 IC50: 0.08 mM Rattus norvegicus 4-nitro-2,1,3-benzothiadiazole
0.095
-
 IC50: 0.095 mM Rattus norvegicus 5-amino-7-methoxy-N1-methylbenzimidazole
0.1
-
 IC50: 0.1 mM Rattus norvegicus 5-nitro-7-methoxybenzimidazole
0.12
-
 IC50: 0.12 mM Rattus norvegicus 2-nitro-1H-benzimidazole
0.15
-
 IC50: 0.15 mM Rattus norvegicus 1-methyl-5-nitro-7-methoxybenzimidazole
0.15
-
 IC50: more than 0.15 mM Rattus norvegicus benzimidazole
0.15
-
 IC50: more than 0.15 mM Rattus norvegicus 1-methylbenzimidazole
0.15
-
 IC50: more than 0.15 mM Rattus norvegicus 5-aminobenzimidazole
0.15
-
 IC50: more than 0.15 mM Rattus norvegicus 5-methoxybenzimidazole
0.15
-
 IC50: more than 0.15 mM Rattus norvegicus 1-methyl-5-nitro-benzimidazole
0.15
-
 IC50: more than 0.15 mM Rattus norvegicus 1-methyl-5-amino-benzimidazole
0.15
-
 IC50: more than 0.15 mM Rattus norvegicus 5-amino-7-methoxybenzimidazole
0.15
-
 IC50: more than 0.15 mM Rattus norvegicus 2,1,3-benzothiadiazole
0.15
-
 IC50: more than 0.15 mM Rattus norvegicus 4-amino-2,1,3-benzothiadiazole