Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 1-20 mM, slight activation | Vigna umbellata |
General Stability | Organism |
---|---|
activity is lost within a week, when the enzyme is frozen or kept at 4°C without glycerol | Vigna umbellata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1L-1,3,4/2,5-cyclohexanepentol | i.e. L-viburnitol | Vigna umbellata | |
2,2'-Anhydro-2-C-hydroxymethyl-myo-inositol | - |
Vigna umbellata | |
2,4,6/3,5-pentahydroxy-cyclohexanone | i.e. myo-inosose-2 | Vigna umbellata | |
Co2+ | 5 mM | Vigna umbellata | |
Cu2+ | 5 mM | Vigna umbellata | |
D,L-2,3,4,6/5-pentahydroxy-cyclohexanone | i.e. myo-inosose-4 | Vigna umbellata | |
EDTA | not EGTA | Vigna umbellata | |
Fe2+ | 5 mM | Vigna umbellata | |
Mn2+ | 5 mM | Vigna umbellata | |
Ni2+ | 5 mM | Vigna umbellata | |
p-chloromercuribenzenesulfonic acid | 0.02 mM | Vigna umbellata | |
S-adenosyl-L-homocysteine | most potent inhibitor | Vigna umbellata | |
Zn2+ | 5 mM | Vigna umbellata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
S-adenosyl-L-methionine | with myo-inositol as cosubstrate | Vigna umbellata | |
0.3 | - |
D,L-2,3,4,6/5-pentahydroxy-cyclohexanone | - |
Vigna umbellata | |
0.67 | - |
2,4,6/3,5-pentahydroxy-cyclohexanone | - |
Vigna umbellata | |
2.92 | - |
myo-inositol | - |
Vigna umbellata | |
4.47 | - |
1-L-1,3,4/2,5-cyclohexanepentol | - |
Vigna umbellata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 5 mM, slight stimulation | Vigna umbellata | |
Na+ | 5 mM, slight stimulation | Vigna umbellata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40300 | - |
2 * or 3 * 40300, SDS-PAGE | Vigna umbellata |
106200 | - |
gel filtration | Vigna umbellata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + myo-inositol | Vigna umbellata | the product 1D-4-O-methyl-myo-inositol is accumulated in response to abiotic stress | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna umbellata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vigna umbellata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | - |
Vigna umbellata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Vigna umbellata |
Storage Stability | Organism |
---|---|
-30°C, 25 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol, 2 mM MgCl2, 0.25 mM ammonium sulfate, 50% glycerol, half-life: 300 d | Vigna umbellata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 1L-1,3,4/2,5-cyclohexanepentol | i.e. L-viburnitol | Vigna umbellata | ? | - |
? | |
S-adenosyl-L-methionine + 2,4,6/3,5-pentahydroxy-cyclohexanone | i.e. myo-inosose-2 | Vigna umbellata | ? | - |
? | |
S-adenosyl-L-methionine + D,L-2,3,4,6/5-pentahydroxy-cyclohexanone | i.e. myo-inosose-4 | Vigna umbellata | ? | - |
? | |
S-adenosyl-L-methionine + myo-inositol | - |
Vigna umbellata | S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol | 1D-4-O-methyl-myo-inositol is accumulated in response to abiotic stresses | ? | |
S-adenosyl-L-methionine + myo-inositol | the product 1D-4-O-methyl-myo-inositol is accumulated in response to abiotic stress | Vigna umbellata | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 2 * or 3 * 40300, SDS-PAGE | Vigna umbellata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Vigna umbellata |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | 30°C: about 40% of maximal activity, 55°C: about 30% of maximal activity | Vigna umbellata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
30 min, stable up to | Vigna umbellata |
50 | - |
30 min, about 40% loss of activity | Vigna umbellata |
60 | - |
30 min, complete loss of activity | Vigna umbellata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Vigna umbellata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.8 | about 35% of maximal activity at pH 7.0 and pH 8.8 | Vigna umbellata |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
3 h, at room temperature, about 45% loss of activity compared to maximal stability at pH 7.0-7.6 | Vigna umbellata |
6.5 | - |
3 h, at room temperature, about 10% loss of activity compared to maximal stability at pH 7.0-7.6 | Vigna umbellata |
7 | 7.6 | 3 h, at room temperature, maximal stability | Vigna umbellata |
8 | - |
3 h, at room temperature, about 30% loss of activity compared to maximal stability at pH 7.0-7.6 | Vigna umbellata |
9 | - |
3 h, at room temperature, about 70% loss of activity compared to maximal stability at pH 7.0-7.6 | Vigna umbellata |