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Literature summary for 2.1.1.129 extracted from

  • Wanek, W.; Richter, A.
    Purification and characterization of myo-inositol 6-O-methyltransferase from Vigna umbellata Ohwi et Ohashi (1995), Planta, 197, 427-434.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 1-20 mM, slight activation Vigna umbellata

General Stability

General Stability Organism
activity is lost within a week, when the enzyme is frozen or kept at 4°C without glycerol Vigna umbellata

Inhibitors

Inhibitors Comment Organism Structure
1L-1,3,4/2,5-cyclohexanepentol i.e. L-viburnitol Vigna umbellata
2,2'-Anhydro-2-C-hydroxymethyl-myo-inositol
-
Vigna umbellata
2,4,6/3,5-pentahydroxy-cyclohexanone i.e. myo-inosose-2 Vigna umbellata
Co2+ 5 mM Vigna umbellata
Cu2+ 5 mM Vigna umbellata
D,L-2,3,4,6/5-pentahydroxy-cyclohexanone i.e. myo-inosose-4 Vigna umbellata
EDTA not EGTA Vigna umbellata
Fe2+ 5 mM Vigna umbellata
Mn2+ 5 mM Vigna umbellata
Ni2+ 5 mM Vigna umbellata
p-chloromercuribenzenesulfonic acid 0.02 mM Vigna umbellata
S-adenosyl-L-homocysteine most potent inhibitor Vigna umbellata
Zn2+ 5 mM Vigna umbellata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
S-adenosyl-L-methionine with myo-inositol as cosubstrate Vigna umbellata
0.3
-
D,L-2,3,4,6/5-pentahydroxy-cyclohexanone
-
Vigna umbellata
0.67
-
2,4,6/3,5-pentahydroxy-cyclohexanone
-
Vigna umbellata
2.92
-
myo-inositol
-
Vigna umbellata
4.47
-
1-L-1,3,4/2,5-cyclohexanepentol
-
Vigna umbellata

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 5 mM, slight stimulation Vigna umbellata
Na+ 5 mM, slight stimulation Vigna umbellata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40300
-
2 * or 3 * 40300, SDS-PAGE Vigna umbellata
106200
-
gel filtration Vigna umbellata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + myo-inositol Vigna umbellata the product 1D-4-O-methyl-myo-inositol is accumulated in response to abiotic stress ?
-
?

Organism

Organism UniProt Comment Textmining
Vigna umbellata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Vigna umbellata

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
Vigna umbellata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Vigna umbellata

Storage Stability

Storage Stability Organism
-30°C, 25 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol, 2 mM MgCl2, 0.25 mM ammonium sulfate, 50% glycerol, half-life: 300 d Vigna umbellata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 1L-1,3,4/2,5-cyclohexanepentol i.e. L-viburnitol Vigna umbellata ?
-
?
S-adenosyl-L-methionine + 2,4,6/3,5-pentahydroxy-cyclohexanone i.e. myo-inosose-2 Vigna umbellata ?
-
?
S-adenosyl-L-methionine + D,L-2,3,4,6/5-pentahydroxy-cyclohexanone i.e. myo-inosose-4 Vigna umbellata ?
-
?
S-adenosyl-L-methionine + myo-inositol
-
Vigna umbellata S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol 1D-4-O-methyl-myo-inositol is accumulated in response to abiotic stresses ?
S-adenosyl-L-methionine + myo-inositol the product 1D-4-O-methyl-myo-inositol is accumulated in response to abiotic stress Vigna umbellata ?
-
?

Subunits

Subunits Comment Organism
? 2 * or 3 * 40300, SDS-PAGE Vigna umbellata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Vigna umbellata

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 30°C: about 40% of maximal activity, 55°C: about 30% of maximal activity Vigna umbellata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
30 min, stable up to Vigna umbellata
50
-
30 min, about 40% loss of activity Vigna umbellata
60
-
30 min, complete loss of activity Vigna umbellata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Vigna umbellata

pH Range

pH Minimum pH Maximum Comment Organism
7 8.8 about 35% of maximal activity at pH 7.0 and pH 8.8 Vigna umbellata

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
3 h, at room temperature, about 45% loss of activity compared to maximal stability at pH 7.0-7.6 Vigna umbellata
6.5
-
3 h, at room temperature, about 10% loss of activity compared to maximal stability at pH 7.0-7.6 Vigna umbellata
7 7.6 3 h, at room temperature, maximal stability Vigna umbellata
8
-
3 h, at room temperature, about 30% loss of activity compared to maximal stability at pH 7.0-7.6 Vigna umbellata
9
-
3 h, at room temperature, about 70% loss of activity compared to maximal stability at pH 7.0-7.6 Vigna umbellata