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Literature summary for 2.1.1.128 extracted from

  • Robin, A.Y.; Giustini, C.; Graindorge, M.; Matringe, M.; Dumas, R.
    Crystal structure of norcoclaurine-6-O-methyltransferase, a key rate-limiting step in the synthesis of benzylisoquinoline alkaloids (2016), Plant J., 87, 641-653 .
    View publication on PubMed
Search for more literature for 2.1.1.128

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thalictrum flavum subsp. glaucum

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of apo-6OMT, the binary complex 6OMT/SAH and the two ternary complexes 6OMT/SAH/norlaudanosoline and 6OMT/SAH/sanguinarine, refined to a resolution of 2.6, 1.9, 1.6 and 1.8 A, respectively Thalictrum flavum subsp. glaucum

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: berberine up to 1 mM Thalictrum flavum subsp. glaucum
sanguinarine slow tight-binding inhibitor Thalictrum flavum subsp. glaucum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 (R,S)-norlaudanosoline pH 8.0, 30°C Thalictrum flavum subsp. glaucum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
 gel filtration, recombinant protein Thalictrum flavum subsp. glaucum

Organism

Organism UniProt Comment Textmining
Thalictrum flavum subsp. glaucum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + (R,S)-norlaudanosoline
-
 Thalictrum flavum subsp. glaucum S-adenosyl-L-homocysteine + (RS)-6-O-methylnorlaudanosoline
-
 ?

Synonyms

Synonyms Comment Organism
6OMT
-
 Thalictrum flavum subsp. glaucum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 (R,S)-norlaudanosoline pH 8.0, 30°C Thalictrum flavum subsp. glaucum