BRENDA - Enzyme Database
show all sequences of 2.1.1.117

S-Adenosyl-L-methionine:scoulerine-9-O-methyltransferase from cultured Coptis japonica cells

Sato, F.; Takeshita, N.; Fitchen, J.H.; Fujiwara, H.; Yamada, Y.; Phytochemistry 32, 659-664 (1993)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Berberine
5 mM, 70% inhibition
Coptis japonica
Ca2+
1 mM, 64% inhibition
Coptis japonica
Cu2+
1 mM, 72% inhibition
Coptis japonica
Mn2+
1 mM, 59% inhibition
Coptis japonica
palmatine
5 mM, 70% inhibition
Coptis japonica
PCMB
0.5 mM, 51% inhibition
Coptis japonica
S-adenosylhomocysteine
-
Coptis japonica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
scoulerine
-
Coptis japonica
0.17
-
S-adenosyl-L-methionine
-
Coptis japonica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
x * 41000, SDS-PAGE
Coptis japonica
120000
140000
gel filtration
Coptis japonica
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Coptis japonica
-
-
-
Purification (Commentary)
Commentary
Organism
-
Coptis japonica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-Adenosyl-L-methionine + (S)-scoulerine
-
15193
Coptis japonica
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
-
-
-
S-Adenosyl-L-methionine + 6-O-methylnorlaudanosoline
8% of the activity relative to (S)-scoulerine
15193
Coptis japonica
?
-
-
-
-
S-Adenosyl-L-methionine + norlaudanosoline
2% of the activity relative to (S)-scoulerine
15193
Coptis japonica
?
-
-
-
-
S-Adenosyl-L-methionine + norreticuline
9% of the activity relative to (S)-scoulerine
15193
Coptis japonica
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 41000, SDS-PAGE
Coptis japonica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
9.5
-
Coptis japonica
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
10
50% of maximal activity at pH 7.5 and at pH 10
Coptis japonica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Berberine
5 mM, 70% inhibition
Coptis japonica
Ca2+
1 mM, 64% inhibition
Coptis japonica
Cu2+
1 mM, 72% inhibition
Coptis japonica
Mn2+
1 mM, 59% inhibition
Coptis japonica
palmatine
5 mM, 70% inhibition
Coptis japonica
PCMB
0.5 mM, 51% inhibition
Coptis japonica
S-adenosylhomocysteine
-
Coptis japonica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
scoulerine
-
Coptis japonica
0.17
-
S-adenosyl-L-methionine
-
Coptis japonica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
x * 41000, SDS-PAGE
Coptis japonica
120000
140000
gel filtration
Coptis japonica
Purification (Commentary) (protein specific)
Commentary
Organism
-
Coptis japonica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-Adenosyl-L-methionine + (S)-scoulerine
-
15193
Coptis japonica
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
-
-
-
S-Adenosyl-L-methionine + 6-O-methylnorlaudanosoline
8% of the activity relative to (S)-scoulerine
15193
Coptis japonica
?
-
-
-
-
S-Adenosyl-L-methionine + norlaudanosoline
2% of the activity relative to (S)-scoulerine
15193
Coptis japonica
?
-
-
-
-
S-Adenosyl-L-methionine + norreticuline
9% of the activity relative to (S)-scoulerine
15193
Coptis japonica
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 41000, SDS-PAGE
Coptis japonica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
9.5
-
Coptis japonica
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
10
50% of maximal activity at pH 7.5 and at pH 10
Coptis japonica
Other publictions for EC 2.1.1.117
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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733669
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720655
Takemura
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Coptis japonica
Plant Cell Physiol.
51
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1
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5
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1
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1
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1
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1
1
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658313
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Transient RNA silencing of sco ...
Coptis japonica
Biosci. Biotechnol. Biochem.
69
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2005
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1
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
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1
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1
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-
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-
15194
Takeshita
Molecular cloning and characte ...
Coptis japonica
Plant Cell Physiol.
36
29-36
1995
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
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-
-
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-
-
1
-
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-
-
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-
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-
1
1
-
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-
-
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-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15192
Fujiwara
-
Expression of (S)-scoulerine 9 ...
Coptis japonica
Phytochemistry
34
949-954
1993
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
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-
-
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4
-
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2
-
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1
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4
-
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2
-
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-
-
-
-
-
-
-
-
-
-
-
-
15193
Sato
-
S-Adenosyl-L-methionine:scoule ...
Coptis japonica
Phytochemistry
32
659-664
1993
-
-
-
-
-
-
7
2
-
-
2
-
-
1
-
-
1
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4
1
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-
1
1
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7
-
2
-
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2
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1
-
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-
4
1
-
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-
-
1
1
-
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-
-
-
-
-
-
15195
Galneder
Alternative final steps in ber ...
Berberis stolonifera, Coptis japonica
Plant Cell Rep.
7
1-4
1988
-
-
-
-
-
-
-
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2
-
2
-
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4
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2
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4
-
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-
-
-
-
-
-
-
-
-
-
-
-
15196
Muemmler
S-Adenosyl-L-methionine:(S)-sc ...
Berberis wilsoniae
Plant Cell Rep.
4
36-39
1985
-
-
-
-
-
-
2
2
-
-
1
1
-
2
-
-
1
-
-
2
1
1
4
-
1
-
-
-
1
-
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-
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-
-
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-
-
-
-
-
-
2
-
2
-
-
1
1
-
-
-
1
-
2
1
1
4
-
1
-
-
-
1
-
-
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-
-
-
-
-
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