Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-3'-Hydroxy-coclaurine | - |
Berberis koetineana | |
(S)-3'-hydroxy-coclaurine | - |
Berberis koetineana | |
(S)-norreticuline | - |
Berberis koetineana | |
(S)-reticuline | - |
Berberis koetineana | |
3'-Hydroxy-N-methyl-(R)-coclaurine | - |
Berberis koetineana | |
Berberine | - |
Berberis koetineana | |
Jatrorrhizine | - |
Berberis koetineana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
3'-hydroxy-N-methyl-(S)-coclaurine | - |
Berberis koetineana | |
0.03 | - |
S-adenosyl-L-methionine | - |
Berberis koetineana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
1 * 40000, SDS-PAGE | Berberis koetineana |
51000 | - |
gel filtration | Berberis koetineana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-Adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine | Berberis koetineana | the enzyme catalyzes the last step in (S)-reticuline biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Berberis koetineana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Berberis koetineana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Berberis koetineana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Berberis koetineana |
Storage Stability | Organism |
---|---|
-20°C, 20% glycerol, stable for more than 1 year | Berberis koetineana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-Adenosyl-L-methionine + (R,S)-7-O-methyllaudanosoline | 93% of the activity relative to 3'-hydroxy-N-methyl-(S)-coclaurine | Berberis koetineana | S-Adenosyl-L-homocysteine + norprotosinomenine | - |
? | |
S-Adenosyl-L-methionine + (R,S)-laudanosoline | 84% of the activity relative to 3'-hydroxy-N-methyl-(S)-coclaurine | Berberis koetineana | S-Adenosyl-L-homocysteine + 4'-O-methyllaudanosoline | - |
? | |
S-Adenosyl-L-methionine + (S)-3'-hydroxy-coclaurine | 10% of the activity relative to 3'-hydroxy-N-methyl-(S)-coclaurine | Berberis koetineana | S-Adenosyl-L-homocysteine + (S)-norreticuline | - |
? | |
S-Adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine | - |
Berberis koetineana | S-Adenosyl-L-homocysteine + (S)-reticuline | - |
? | |
S-Adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine | the enzyme catalyzes the last step in (S)-reticuline biosynthesis | Berberis koetineana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Berberis koetineana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Berberis koetineana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
6 | - |
half-life: 15 days | Berberis koetineana |
20 | - |
half-life: 3 days | Berberis koetineana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | 8.5 | - |
Berberis koetineana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | about 50% of maximal activity at pH 7 and at pH 9 | Berberis koetineana |