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Literature summary for 2.1.1.115 extracted from

  • Frenzel, T.; Zenk, M.H.
    Purification and characterization of three isoforms of S-adenosyl-L-methionine: (R,S)-tetrahydrobenzylisoquinoline-N-methyltransferase from Berberis koetineana cell cultures (1990), Phytochemistry, 29, 3491-3497.
No PubMed abstract available
Search for more literature for 2.1.1.115

Application

Application Comment Organism
synthesis the enzyme immobilized on CH-Sepharose or CPG-10 glass beads is a useful tool for the preparative synthesis of isotopically labelled N-methylated benzylisoquinoline alkaloids Berberis koetineana

General Stability

General Stability Organism
-20°C, in presence of 20% glycerol, half-life of immobilized enzyme: 40 days Berberis koetineana
6°C, in presence of 20% glycerol, half-life of immobilized enzyme: 20 days Berberis koetineana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
 (S)-6-O-methylnorlaudanosoline isoenzyme NMT-III Berberis koetineana
0.003
-
 (S)-6-O-methylnorlaudanosoline isoenzyme NMT-II Berberis koetineana
0.004
-
 (R)-6-O-methylnorlaudanosoline isoenzyme NMT-II Berberis koetineana
0.005
-
 (S)-norreticuline isoenzyme NMT-III Berberis koetineana
0.005
-
 (S)-norreticuline isoenzyme NMT-I Berberis koetineana
0.009
-
 (S)-norreticuline isoenzyme NMT-II Berberis koetineana
0.012
-
 (R)-norreticuline isoenzyme NMT-III Berberis koetineana
0.012
-
 (R)-norreticuline isoenzyme NMT-I Berberis koetineana
0.015
-
 (R)-norreticuline isoenzyme NMT-II Berberis koetineana
0.017
-
 (S)-norreticuline isoenzyme NMT-I Berberis koetineana
0.018
-
 (R)-norreticuline isoenzyme NMT-I Berberis koetineana
0.05
-
 (R)-6-O-methylnorlaudanosoline isoenzyme NMT-III Berberis koetineana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 isoenzyme NMT-I and NMT-III, gel filtration Berberis koetineana
68000
-
 gel filtration Berberis vulgaris
78000
-
 isoenzyme NMT-II, gel filtration Berberis koetineana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-Adenosyl-L-methionine + coclaurine Berberis koetineana
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Argemone platyceras
-
-
-
Berberis angulosa
-
-
-
Berberis beaniana
-
-
-
Berberis bretschneideri
-
-
-
Berberis carminea
-
-
-
Berberis crataegina
-
-
-
Berberis dictyophylla
-
-
-
Berberis gagnepainii
-
-
-
Berberis henryana
-
-
-
Berberis julianae
-
-
-
Berberis koetineana
-
 3 isoenzymes: NMT-I, NMT-II, NMT-III
-
Berberis laxifolia
-
-
-
Berberis nervosa
-
-
-
Berberis stenophylla
-
-
-
Berberis stolonifera
-
 V 29
-
Berberis stolonifera V 29
-
 V 29
-
Berberis taliensis
-
-
-
Berberis thunbergii
-
 Atropurpurea
-
Berberis turcomanica
-
-
-
Berberis vulgaris
-
-
-
Berberis wilsoniae
-
-
-
Bocconia cordata
-
-
-
Coptis japonica
-
-
-
Corydalis vaginans
-
-
-
Eschscholzia californica
-
-
-
Glaucium corniculatum
-
-
-
Glaucium elegans
-
-
-
Glaucium flavum
-
-
-
Hyalosepalum caffrum
-
-
-
Podophyllum peltatum
-
-
-
Sinopodophyllum hexandrum
-
-
-
Stephania delavayi
-
-
-
Thalictrum dipterocarpum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Berberis koetineana

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Berberis koetineana
-

Storage Stability

Storage Stability Organism
-20°C, in presence of 20% glycerol, half-life of immobilized enzyme: 40 days Berberis koetineana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-Adenosyl-L-methionine + (R)-6-O-methylnorlaudanosoline
-
 Berberis koetineana ?
-
 ?
S-Adenosyl-L-methionine + (R)-norcoclaurine
-
 Berberis koetineana ?
-
 ?
S-Adenosyl-L-methionine + (R)-norprotosinomenine isoenzyme NMT-I: 6% of maximal activity with (R)-tetrahydropapaverine Berberis koetineana ?
-
 ?
S-Adenosyl-L-methionine + (R,S)-4'-O-methylnorlaudanosoline isoenzyme NMT-I: 3% of maximal activity with (R)-tetrahydropapaverine Berberis koetineana ?
-
 ?
S-Adenosyl-L-methionine + (R,S)-7-O-methylnorlaudanosoline isoenzyme NMT-I: 12% of maximal activity with (R)-tetrahydropapaverine Berberis koetineana ?
-
 ?
S-Adenosyl-L-methionine + (R,S)-isococlaurine
-
 Berberis koetineana ?
-
 ?
S-Adenosyl-L-methionine + (R,S)-norarmepavine
-
 Berberis koetineana ?
-
 ?
S-Adenosyl-L-methionine + (R,S)-norcodamine
-
 Berberis koetineana ?
-
 ?
S-Adenosyl-L-methionine + (R,S)-nororientaline
-
 Berberis koetineana ?
-
 ?
S-Adenosyl-L-methionine + (RS)-1-benzyl-1,2,3,4-tetrahydroisoquinoline
-
 Berberis koetineana ?
-
 ?
S-Adenosyl-L-methionine + (S)-6-O-methylnorlaudanosoline
-
 Berberis koetineana ?
-
 ?
S-Adenosyl-L-methionine + (S)-norcoclaurine
-
 Berberis koetineana ?
-
 ?
S-Adenosyl-L-methionine + coclaurine
-
 Berberis koetineana ?
-
 ?
S-Adenosylmethionine + (R)-coclaurine isoenzyme NMT-II and NMT-III show maximal activity with (R)-coclaurine Berberis koetineana S-Adenosyl-L-homocysteine + N-methyl-(RS)-1-benzyl-1,2,3,4-tetrahydroisoquinoline
-
 ?
S-Adenosylmethionine + (R)-norreticuline
-
 Berberis koetineana ?
-
 ?
S-Adenosylmethionine + (R)-tetrahydropapaverine isoenzyme NMT-I shows maximal activity with (R)-tetrahydropapaverine Berberis koetineana ?
-
 ?
S-Adenosylmethionine + (S)-coclaurine
-
 Berberis koetineana S-Adenosylhomocysteine + N-methylcoclaurine
-
 ?
S-Adenosylmethionine + (S)-norreticuline
-
 Berberis koetineana S-Adenosylhomocysteine + (S)-scoulerine
-
 ?
S-Adenosylmethionine + (S)-tetrahydropapaverine
-
 Berberis koetineana ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40 isoenzyme NMT-I, NMT-II and NMT-III Berberis koetineana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6
-
 in presence of 20% glycerol, half-life of immobilized enzyme: 20 days Berberis koetineana
20
-
 in presence of 20% glycerol, half-life of immobilized enzyme: 7 days Berberis koetineana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 isoenzyme NMT-III Berberis koetineana
7.4
-
 isoenzyme NMT-I and NMT-II Berberis koetineana