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Literature summary for 2.1.1.113 extracted from
- Methylation by a unique alpha-class N4-cytosine methyltransferase is required for DNA transformation of Caldicellulosiruptor bescii DSM6725 (2012), PLoS ONE, 7, e43844.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells | Caldicellulosiruptor bescii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33000, SDS-PAGE | Caldicellulosiruptor bescii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + DNA cytosine | Caldicellulosiruptor bescii | - |
S-adenosyl-L-homocysteine + DNA N4-methylcytosine | - |
? |  |
| S-adenosyl-L-methionine + DNA cytosine | Caldicellulosiruptor bescii DSM 6725 | - |
S-adenosyl-L-homocysteine + DNA N4-methylcytosine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor bescii | B9MK94 | - |
- |
| Caldicellulosiruptor bescii DSM 6725 | B9MK94 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Caldicellulosiruptor bescii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + DNA cytosine | - |
Caldicellulosiruptor bescii | S-adenosyl-L-homocysteine + DNA N4-methylcytosine | - |
? | |
| S-adenosyl-L-methionine + DNA cytosine | - |
Caldicellulosiruptor bescii DSM 6725 | S-adenosyl-L-homocysteine + DNA N4-methylcytosine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, SDS-PAGE | Caldicellulosiruptor bescii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| M.CbeI | - |
Caldicellulosiruptor bescii |
| N4-cytosine methyltransferase | - |
Caldicellulosiruptor bescii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | cytosine N4-methylation is required for DNA transformation | Caldicellulosiruptor bescii |
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Release 2023.1 (January 2023)
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