KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
sterigmatocystin | - |
Aspergillus parasiticus | |
0.0096 | - |
S-adenosyl-L-methionine | - |
Aspergillus parasiticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Aspergillus parasiticus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SRRC 163, SDS-PAGE, the 40000 Da protein is distinct from the 168000 Da methyl transferase purified from the same fungal strain | Aspergillus parasiticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + sterigmatocystin | Aspergillus flavus | penultimate step in aflatoxin biosynthetic pathway | S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin | - |
? | |
S-adenosyl-L-methionine + sterigmatocystin | Aspergillus parasiticus | penultimate step in aflatoxin biosynthetic pathway | S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin | - |
? | |
S-adenosyl-L-methionine + sterigmatocystin | Aspergillus parasiticus SRCC 163 | penultimate step in aflatoxin biosynthetic pathway | S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus flavus | - |
- |
- |
Aspergillus parasiticus | - |
SRCC 163 | - |
Aspergillus parasiticus SRCC 163 | - |
SRCC 163 | - |
Purification (Comment) | Organism |
---|---|
strain SRRC 163, anion exchange, hydroxylapatite, DEAE-Sperodex, octyl avidgel, anion-exchange | Aspergillus parasiticus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus flavus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.021 | - |
- |
Aspergillus parasiticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + sterigmatocystin | - |
Aspergillus flavus | S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin | - |
? | |
S-adenosyl-L-methionine + sterigmatocystin | - |
Aspergillus parasiticus | S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin | - |
? | |
S-adenosyl-L-methionine + sterigmatocystin | penultimate step in aflatoxin biosynthetic pathway | Aspergillus flavus | S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin | - |
? | |
S-adenosyl-L-methionine + sterigmatocystin | penultimate step in aflatoxin biosynthetic pathway | Aspergillus parasiticus | S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin | - |
? | |
S-adenosyl-L-methionine + sterigmatocystin | - |
Aspergillus parasiticus SRCC 163 | S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin | - |
? | |
S-adenosyl-L-methionine + sterigmatocystin | penultimate step in aflatoxin biosynthetic pathway | Aspergillus parasiticus SRCC 163 | S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SRRC 163, SDS-PAGE, the 40000 Da protein is distinct from the 168000 Da methyl transferase purified from the same fungal strain | Aspergillus parasiticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Aspergillus parasiticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | approx. 35% of maximal activity at 25°C, approx. 30% of maximal activity at 60°C | Aspergillus parasiticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
sterigmatocystin | - |
Aspergillus parasiticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9.4 | - |
Aspergillus parasiticus |