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Literature summary for 2.1.1.11 extracted from

  • McLean, S.; Hunter, C.N.
    An enzyme-coupled continuous spectrophotometric assay for magnesium protoporphyrin IX methyltransferases (2009), Anal. Biochem., 394, 223-228.
    View publication on PubMed

Application

Application Comment Organism
analysis a continuous enzyme-coupled spectrophotometric assay for ChlM from Synechocystis sp. PCC 6803 is reported Synechocystis sp. PCC 6803

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET9a for expression in Escherichia coli BL21DE3 cells Synechocystis sp. PCC 6803

Metals/Ions

Metals/Ions Comment Organism Structure
Magnesium
-
Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + magnesium protoporphyrin IX Synechocystis sp. PCC 6803
-
S-adenosyl-L-homocysteine + magnesium protoporphyrin IX 13-methyl ester
-
?

Organism

Organism UniProt Comment Textmining
Synechocystis sp. PCC 6803
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a chelating Sepharose Fast Flow resin charged with 50 mM NiSO4 Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + magnesium deuteroporphyrin IX activity assay Synechocystis sp. PCC 6803 S-adenosyl-L-homocysteine + magnesium deuteroporphyrin IX 13-methyl ester
-
?
S-adenosyl-L-methionine + magnesium protoporphyrin IX
-
Synechocystis sp. PCC 6803 S-adenosyl-L-homocysteine + magnesium protoporphyrin IX 13-methyl ester
-
?

Synonyms

Synonyms Comment Organism
ChlM
-
Synechocystis sp. PCC 6803
magnesium protoporphyrin IX methyltransferase
-
Synechocystis sp. PCC 6803

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
activity assay Synechocystis sp. PCC 6803

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
continuous spectrophotometric assay Synechocystis sp. PCC 6803
7.5
-
HPLC-based assay Synechocystis sp. PCC 6803

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Synechocystis sp. PCC 6803

Expression

Organism Comment Expression
Synechocystis sp. PCC 6803 the H subunit of Mg chelatase, ChlH, stimulates ChlM activity up