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Literature summary for 2.1.1.11 extracted from

  • Pontier, D.; Albrieux, C.; Joyard, J.; Lagrange, T.; Block, M.A.
    Knock-out of the magnesium protoporphyrin IX methyltransferase gene in Arabidopsis. Effects on chloroplast development and on chloroplast-to-nucleus signaling (2007), J. Biol. Chem., 282, 2297-2304.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Arabidopsis thaliana
expression in Escherichia coli, pCambia derivative, binary vector introduced into the GV3121 strain of Agrobacterium tumefaciens, transgenic plants generated, EYFP fusion protein for localization studies, insertion of the T-DNA in single intron present in the coding sequence, deletion of the 5' part of the gene including the entire first exon, null-mutant Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-
plastid CHLH protein matured and accumulated inside plastids Arabidopsis thaliana 9536
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
144000
-
mature protein, SDS-PAGE, mutant and wild-type Arabidopsis thaliana
154000
-
unprocessed precursor protein, mutant Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + magnesium protoporphyrin Arabidopsis thaliana
-
S-adenosyl-L-homocysteine + magnesium protoporphyrin monomethyl ester
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana
-
chlm mutant (ABN42 line) of the ecotype Wassilewskija (WS)
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification processing of transit peptide during import into chloroplast Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
chelating Sepharose fast-flow resin column chromatography and P-6 desalting gel filtration Arabidopsis thaliana
recombinant protein, gel filtration and SDS-PAGE Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon 8 day plants, different light conditions Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
leaf chlorophyll fluorescence Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
knock-out mutant characterized, tetrapyrole intermediate analysis, RT-PCR and immunoblot analysis, CHLM gene essential for formation of chlorophyll, photosystems I and II and cyt b6f complexes, negative effector of nuclear photosynthetic gene expression, product of CHLM possibly involved in retrograde signaling, post-transcriptional up-regulation in chlm mutants, specific accumulation inside plastids determined, regulatory role of CHLH subunit Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + magnesium protoporphyrin
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + magnesium protoporphyrin monomethyl ester
-
?
S-adenosyl-L-methionine + magnesium protoporphyrin key gene for chlorophyll biosynthesis and chloroplast development, reaction products probably involved in retrograde chloroplast-to-nucleus signalling Arabidopsis thaliana S-adenosyl-L-homocysteine + magnesium protoporphyrin monomethyl ester
-
?

Synonyms

Synonyms Comment Organism
ChlM
-
Arabidopsis thaliana