Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Arabidopsis thaliana |
expression in Escherichia coli, pCambia derivative, binary vector introduced into the GV3121 strain of Agrobacterium tumefaciens, transgenic plants generated, EYFP fusion protein for localization studies, insertion of the T-DNA in single intron present in the coding sequence, deletion of the 5' part of the gene including the entire first exon, null-mutant | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
plastid | CHLH protein matured and accumulated inside plastids | Arabidopsis thaliana | 9536 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
144000 | - |
mature protein, SDS-PAGE, mutant and wild-type | Arabidopsis thaliana |
154000 | - |
unprocessed precursor protein, mutant | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + magnesium protoporphyrin | Arabidopsis thaliana | - |
S-adenosyl-L-homocysteine + magnesium protoporphyrin monomethyl ester | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | - |
chlm mutant (ABN42 line) of the ecotype Wassilewskija (WS) | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | processing of transit peptide during import into chloroplast | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
chelating Sepharose fast-flow resin column chromatography and P-6 desalting gel filtration | Arabidopsis thaliana |
recombinant protein, gel filtration and SDS-PAGE | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | 8 day plants, different light conditions | Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
leaf | chlorophyll fluorescence | Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
knock-out mutant characterized, tetrapyrole intermediate analysis, RT-PCR and immunoblot analysis, CHLM gene essential for formation of chlorophyll, photosystems I and II and cyt b6f complexes, negative effector of nuclear photosynthetic gene expression, product of CHLM possibly involved in retrograde signaling, post-transcriptional up-regulation in chlm mutants, specific accumulation inside plastids determined, regulatory role of CHLH subunit | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + magnesium protoporphyrin | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + magnesium protoporphyrin monomethyl ester | - |
? | |
S-adenosyl-L-methionine + magnesium protoporphyrin | key gene for chlorophyll biosynthesis and chloroplast development, reaction products probably involved in retrograde chloroplast-to-nucleus signalling | Arabidopsis thaliana | S-adenosyl-L-homocysteine + magnesium protoporphyrin monomethyl ester | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ChlM | - |
Arabidopsis thaliana |