Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.108 extracted from

  • Kurosaki, F.
    Partial purification and characterization of 6-hydroxymellein-O-methyltransferase from elicitor-treated carrot cells (1996), Phytochemistry, 41, 1023-1027.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
6-methoxymellein
-
Daucus carota
Co2+
-
Daucus carota
Cu2+
-
Daucus carota
Fe2+
-
Daucus carota
Hg2+
-
Daucus carota
iodoacetoamide
-
Daucus carota
Mn2+
-
Daucus carota
p-chloromercuribenzoate
-
Daucus carota
S-adenosyl-L-homocysteine
-
Daucus carota
sulfhydryl reagents
-
Daucus carota

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
gel filtration Daucus carota

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 6-hydroxymellein Daucus carota enzyme is involved in the biosynthesis of the phytoalexin 6-methoxymellein in carrot tissue S-adenosyl-L-homocysteine + 6-methoxymellein
-
?
S-adenosyl-L-methionine + 6-hydroxymellein Daucus carota biosynthetic pathway S-adenosyl-L-homocysteine + 6-methoxymellein
-
?

Organism

Organism UniProt Comment Textmining
Daucus carota
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Daucus carota

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + 6-hydroxymellein = S-adenosyl-L-homocysteine + 6-methoxymellein mechanism Daucus carota

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Daucus carota

Storage Stability

Storage Stability Organism
4°C, 7 d, complete loss of activity Daucus carota
frozen overnight, complete loss of activity Daucus carota

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 6-hydroxymellein
-
Daucus carota S-adenosyl-L-homocysteine + 6-methoxymellein
-
?
S-adenosyl-L-methionine + 6-hydroxymellein enzyme is involved in the biosynthesis of the phytoalexin 6-methoxymellein in carrot tissue Daucus carota S-adenosyl-L-homocysteine + 6-methoxymellein
-
?
S-adenosyl-L-methionine + 6-hydroxymellein biosynthetic pathway Daucus carota S-adenosyl-L-homocysteine + 6-methoxymellein
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 pI: 5.7 Daucus carota

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.026
-
S-adenosyl-L-homocysteine
-
Daucus carota
0.047
-
6-methoxymellein
-
Daucus carota