Application | Comment | Organism |
---|---|---|
analysis | red fluorescent compounds are associated with the recombinant mature SUMT which are identified as sirohydrochlorin and trimethylpyrrocorphin by spectroscopic analysis, slightly alter the protein secondary structure | Zea mays |
Cloned (Comment) | Organism |
---|---|
Escherichia coli JM109 used for cloning all recombinant plasmids and expressing the pUC18-derived plasmid, Escherichia coli strain S13009 and BL21(DE3) used for expressing the pQE-derived plasmids and the pET-derived plasmid | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion mutants, truncated one deleting the C-terminal extra 52 amino acids actively expressed in Escherichia coli, the mature SUMT fusion mutant or the mature SUMT deleting the N-terminal 36 amino acids including glycine-rich region involved directly in SAM binding expressed as an inclusion body in Escherichia coli | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000, SDS-PAGE | Zea mays |
52000 | - |
gel filtration | Zea mays |
79000 | - |
dynamic light scattering assay | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by NiNTA column | Zea mays |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 34000, SDS-PAGE | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase | - |
Zea mays |
SUMT | - |
Zea mays |
uroporphyrinogen III methyltransferase | - |
Zea mays |