Cloned (Comment) | Organism |
---|---|
gene TaCCoAOMT1 is located in chromosome 7A, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of the enzyme in Escherichia coli strain BL21(DE3) | Triticum aestivum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00223 | - |
5-hydroxy-feruloyl-CoA | pH 7.5, 30°C, recombinant enzyme | Triticum aestivum | |
0.1123 | - |
caffeoyl-CoA | pH 7.5, 30°C, recombinant enzyme | Triticum aestivum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA | Triticum aestivum | - |
S-adenosyl-L-homocysteine + sinapoyl-CoA | - |
? | |
S-adenosyl-L-methionine + caffeoyl-CoA | Triticum aestivum | - |
S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | - |
wheat lodging-resistant (H4546) and lodging-sensitive (C6001) cultivars | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | lower expression level | Triticum aestivum | - |
root | high expression level | Triticum aestivum | - |
stem | high expression level | Triticum aestivum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | recombinant TaCCoAOMT1 protein can only use caffeoyl-CoA and 5-hydroxyferuloyl-CoA as effective substrates and caffeoyl-CoA as the best substrate. No activity with p-coumarate, caffeate, ferulate, 5-hydroxyferulate, sinapate, coumarylaldehyde, caffeoyaldehyde, coniferaldehyde, 5-hydroxyconiferaldehyde, and sinapaldehyde, 4-coumaryl alcohol, caffeoyl alcohol, coniferyl alcohol, 5-hydroxyconiferyl alcohol, and sinapyl alcohol | Triticum aestivum | ? | - |
- |
|
S-adenosyl-L-methionine + 4-coumaroyl-CoA | very low activity | Triticum aestivum | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA | - |
Triticum aestivum | S-adenosyl-L-homocysteine + sinapoyl-CoA | - |
? | |
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA | 56% activity compared to caffeoyl-CoA | Triticum aestivum | S-adenosyl-L-homocysteine + sinapoyl-CoA | - |
? | |
S-adenosyl-L-methionine + caffeoyl-CoA | - |
Triticum aestivum | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? | |
S-adenosyl-L-methionine + caffeoyl-CoA | best substrate | Triticum aestivum | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? | |
S-adenosyl-L-methionine + feruloyl-CoA | very low activity | Triticum aestivum | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + sinapoyl-CoA | low activity | Triticum aestivum | S-adenosyl-L-homocysteine + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28802, sequence calculation | Triticum aestivum |
Synonyms | Comment | Organism |
---|---|---|
caffeoyl coenzyme A 3-O-methyltransferase | - |
Triticum aestivum |
CCoAOMT | - |
Triticum aestivum |
TaCCoAOMT1 | - |
Triticum aestivum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Triticum aestivum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 35 | over 50% of maximal activity within this range, profile overview | Triticum aestivum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Triticum aestivum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | the enzyme has a very narrow pH range, it is most active at pH 7.5, and inactive below pH 6.5 and above pH 9.5 | Triticum aestivum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Triticum aestivum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Triticum aestivum | sequence calculation | - |
4.9 |
General Information | Comment | Organism |
---|---|---|
physiological function | TaCCoAOMT1 is an important CCoAOMT for lignin biosynthesis that is critical for stem development, but not directly associated with lodging-resistant trait in wheat | Triticum aestivum |