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Literature summary for 2.1.1.104 extracted from

  • Giordano, D.; Provenzano, S.; Ferrandino, A.; Vitali, M.; Pagliarani, C.; Roman, F.; Cardinale, F.; Castellarin, S.D.; Schubert, A.
    Characterization of a multifunctional caffeoyl-CoA O-methyltransferase activated in grape berries upon drought stress (2016), Plant Physiol. Biochem., 101, 23-32 .
    View publication on PubMed
Search for more literature for 2.1.1.104

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Vitis vinifera

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Vitis vinifera
Mn2+
-
 Vitis vinifera
Zn2+
-
 Vitis vinifera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0827
-
 cyanidin 3-O-glucoside thrombin-cleaved enzyme, at pH 8.5 and 30°C Vitis vinifera
0.096
-
 caffeoyl-CoA GST-tagged recombinant enzyme, at pH 8.5 and 30°C Vitis vinifera
0.11
-
 cyanidin 3-O-glucoside GST-tagged recombinant enzyme, at pH 8.5 and 30°C Vitis vinifera

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 5 mM used in assay conditions Vitis vinifera

Organism

Organism UniProt Comment Textmining
Vitis vinifera Q43237 cultivar Barbera
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography Vitis vinifera

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Vitis vinifera
-
leaf
-
 Vitis vinifera
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + caffeoyl-CoA
-
 Vitis vinifera S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
S-adenosyl-L-methionine + cyanidin 3-O-glucoside
-
 Vitis vinifera S-adenosyl-L-homocysteine + peonidin 3-O-glucoside
-
 ?

Synonyms

Synonyms Comment Organism
CCoAOMT
-
 Vitis vinifera
COMT
-
 Vitis vinifera

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0027
-
 cyanidin 3-O-glucoside GST-tagged recombinant enzyme, at pH 8.5 and 30°C Vitis vinifera
0.0082
-
 caffeoyl-CoA GST-tagged recombinant enzyme, at pH 8.5 and 30°C Vitis vinifera
0.0129
-
 cyanidin 3-O-glucoside thrombin-cleaved enzyme, at pH 8.5 and 30°C Vitis vinifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 optimal pH value for the methylation of cyanidin 3-O-glucoside Vitis vinifera

Expression

Organism Comment Expression
Vitis vinifera transcript accumulation of the enzyme is detected in berry skins, and increases during berry ripening on the plant, and in cultured berries treated with abscisic acid. Drought stress increases the enzyme transcript in berries up

General Information

General Information Comment Organism
physiological function the enzyme contributes to anthocyanin methylation activity in grape berries, in particular under drought stress Vitis vinifera