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Literature summary for 2.1.1.104 extracted from

  • Walker, A.M.; Sattler, S.A.; Regner, M.; Jones, J.P.; Ralph, J.; Vermerris, W.; Sattler, S.E.; Kang, C.
    The structure and catalytic mechanism of Sorghum bicolor caffeoyl-CoA O-methyltransferase (2016), Plant Physiol., 172, 78-92 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.104

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta cells Sorghum bicolor

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with S-adenosyl-L-methionine, hanging drop vapor diffusion method, using 0.2 M calcium acetate hydrate, 0.1 M Tris, pH 8.0, and 20% (w/v) PEG 4000 Sorghum bicolor

Protein Variants

Protein Variants Comment Organism
D252A inactive Sorghum bicolor
K180A inactive Sorghum bicolor

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ contains Ca2+ Sorghum bicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + caffeoyl-CoA Sorghum bicolor
-
 S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Sorghum bicolor C5Z4W3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Resource-Q column chromatography and hydroxyapatite column chromatography Sorghum bicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with caffeic acid, 5-hydroxyferuloyl-CoA, feruloyl-CoA, 4-coumaroyl-CoA, and 5-hydroxyferuloyl-CoA Sorghum bicolor ?
-
-
S-adenosyl-L-methionine + caffeoyl-CoA
-
 Sorghum bicolor S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Sorghum bicolor

Synonyms

Synonyms Comment Organism
CCoAOMT
-
 Sorghum bicolor