Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.104 extracted from

  • Brandt, W.; Manke, K.; Vogt, T.
    A catalytic triad - Lys-Asn-Asp - is essential for the catalysis of the methyl transfer in plant cation-dependent O-methyltransferases (2015), Phytochemistry, 113, 130-139.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Mesembryanthemum crystallinum

Protein Variants

Protein Variants Comment Organism
D228A only residual enzyme activities are detectable Mesembryanthemum crystallinum
K157A mutation decreases Km but not the kcat value of eriodictyol, a single, unusual product is formed, namely the yellow fluorescing quercetagetin 5-O-methyl ether Mesembryanthemum crystallinum
N181A complete loss of activity with eriodictyol Mesembryanthemum crystallinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00033
-
quercetagetin wild-type, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
0.0014
-
quercetagetin mutant D228A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
0.003
-
eriodictyol wild-type, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
0.0098
-
quercetagetin mutant N181A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
0.0225
-
eriodictyol mutant D228A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
0.184
-
eriodictyol mutant K157A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
0.249
-
quercetagetin mutant K157A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum

Organism

Organism UniProt Comment Textmining
Mesembryanthemum crystallinum Q6YI95
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information a catalytic triad, consisting of Lys157-Asn181-Asp228 residues is required for complete methyl transfer in case of a cation-dependent phenylpropanoid and flavonoid O-methyl transferase. This triad appears essential for efficient methyl transfer to catechol-like hydroxyl group in phenolics. The triad is conserved among all characterized plant caffeoyl coenzyme A O-methyltransferase-like enzymes Mesembryanthemum crystallinum ?
-
?
S-adenosyl-L-methionine + eriodictyol
-
Mesembryanthemum crystallinum S-adenosyl-L-homocysteine + homoeriodictyol single product ?
S-adenosyl-L-methionine + quercetagetin
-
Mesembryanthemum crystallinum S-adenosyl-L-homocysteine + quercetagetin 6,3'-di-O-methylether
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0016
-
eriodictyol mutant K157A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
0.0021
-
quercetagetin mutant N181A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
0.0028
-
eriodictyol mutant D228A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
0.0116
-
quercetagetin mutant D228A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
0.078
-
quercetagetin wild-type, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
0.1
-
eriodictyol wild-type, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
0.106
-
quercetagetin mutant K157A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0086
-
eriodictyol mutant K157A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
0.123
-
eriodictyol mutant D228A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
0.214
-
quercetagetin mutant N181A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
0.426
-
quercetagetin mutant K157A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
8.285
-
quercetagetin mutant D228A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
33.2
-
eriodictyol wild-type, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
237
-
quercetagetin wild-type, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum