Cloned (Comment) | Organism |
---|---|
- |
Mesembryanthemum crystallinum |
Protein Variants | Comment | Organism |
---|---|---|
D228A | only residual enzyme activities are detectable | Mesembryanthemum crystallinum |
K157A | mutation decreases Km but not the kcat value of eriodictyol, a single, unusual product is formed, namely the yellow fluorescing quercetagetin 5-O-methyl ether | Mesembryanthemum crystallinum |
N181A | complete loss of activity with eriodictyol | Mesembryanthemum crystallinum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00033 | - |
quercetagetin | wild-type, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
0.0014 | - |
quercetagetin | mutant D228A, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
0.003 | - |
eriodictyol | wild-type, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
0.0098 | - |
quercetagetin | mutant N181A, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
0.0225 | - |
eriodictyol | mutant D228A, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
0.184 | - |
eriodictyol | mutant K157A, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
0.249 | - |
quercetagetin | mutant K157A, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesembryanthemum crystallinum | Q6YI95 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | a catalytic triad, consisting of Lys157-Asn181-Asp228 residues is required for complete methyl transfer in case of a cation-dependent phenylpropanoid and flavonoid O-methyl transferase. This triad appears essential for efficient methyl transfer to catechol-like hydroxyl group in phenolics. The triad is conserved among all characterized plant caffeoyl coenzyme A O-methyltransferase-like enzymes | Mesembryanthemum crystallinum | ? | - |
? | |
S-adenosyl-L-methionine + eriodictyol | - |
Mesembryanthemum crystallinum | S-adenosyl-L-homocysteine + homoeriodictyol | single product | ? | |
S-adenosyl-L-methionine + quercetagetin | - |
Mesembryanthemum crystallinum | S-adenosyl-L-homocysteine + quercetagetin 6,3'-di-O-methylether | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
eriodictyol | mutant K157A, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
0.0021 | - |
quercetagetin | mutant N181A, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
0.0028 | - |
eriodictyol | mutant D228A, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
0.0116 | - |
quercetagetin | mutant D228A, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
0.078 | - |
quercetagetin | wild-type, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
0.1 | - |
eriodictyol | wild-type, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
0.106 | - |
quercetagetin | mutant K157A, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0086 | - |
eriodictyol | mutant K157A, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
0.123 | - |
eriodictyol | mutant D228A, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
0.214 | - |
quercetagetin | mutant N181A, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
0.426 | - |
quercetagetin | mutant K157A, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
8.285 | - |
quercetagetin | mutant D228A, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
33.2 | - |
eriodictyol | wild-type, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum | |
237 | - |
quercetagetin | wild-type, pH 7.5, temperature not specified in the publication | Mesembryanthemum crystallinum |