Application | Comment | Organism |
---|---|---|
medicine | inhibition of the enzyme results in Ras mislocalization and loss of cellular transformation ability, making it an attractive and novel anticancer target | Homo sapiens |
medicine | inhibition of the enzyme results in Ras mislocalization and loss of cellular transformation ability, making it an attractive and novel anticancer target | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
His-Ste14p, which encodes Ste14p with a 10 x histidine tag followed by a triply iterated myc epitope repeat at the N terminus under the constitutive control of the phosphoglycerate kinase promoter, overexpressed in Saccharomyces cerevisiae | Saccharomyces cerevisiae |
into the pCHH10m3N vector, overexpressed in Saccharomyces cerevisiae | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-Acetyl-S-(3-(3-methylbut-2-enyl)-5-(4-phenyl)phenylpent-2Z-en-1-yl)-L-cysteine | shows a mix of competitive and uncompetitive inhibition | Homo sapiens | |
N-Acetyl-S-(3-(3-methylbut-2-enyl)-5-(4-phenyl)phenylpent-2Z-en-1-yl)-L-cysteine | shows a mix of competitive and uncompetitive inhibition | Saccharomyces cerevisiae | |
N-acetyl-S-(3-(3-methylbut-2-enyl)-7,11-dimethyldodeca-2Z,6E,10-trien-1-yl)-L-cysteine | competitive inhibitor of overexpressed yeast Icmt | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
membrane | - |
Saccharomyces cerevisiae | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + N-acetyl-S-(3-(3-methylbut-2-enyl)-7,11-dimethyldodeca-2Z,6E,10-trien-1-yl)-L-cysteine | relatively good substrate for the human Icmt enzyme | Homo sapiens | S-adenosyl-L-homocysteine + N-acetyl-S-(3-(3-methylbut-2-enyl)-7,11-dimethyldodeca-2Z,6E,10-trien-1-yl)-L-cysteine methyl ester | - |
? | |
S-adenosyl-L-methionine + N-acetyl-S-farnesyl-L-cysteine | isoprenoid moiety as a key substrate recognition element for Icmt | Homo sapiens | S-adenosyl-L-homocysteine + N-acetyl-S-farnesyl-L-cysteine methyl ester | - |
? | |
S-adenosyl-L-methionine + N-acetyl-S-farnesyl-L-cysteine | isoprenoid moiety as a key substrate recognition element for Icmt | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N-acetyl-S-farnesyl-L-cysteine methyl ester | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Icmt | - |
Homo sapiens |
isoprenylcysteine carboxylmethyltransferase | - |
Homo sapiens |
isoprenylcysteine carboxylmethyltransferase | - |
Saccharomyces cerevisiae |
Ste14p | - |
Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0171 | - |
N-acetyl-S-(3-(3-methylbut-2-enyl)-7,11-dimethyldodeca-2Z,6E,10-trien-1-yl)-L-cysteine | - |
Saccharomyces cerevisiae | |
0.0354 | - |
N-Acetyl-S-(3-(3-methylbut-2-enyl)-5-(4-phenyl)phenylpent-2Z-en-1-yl)-L-cysteine | competitive inhibition | Saccharomyces cerevisiae | |
0.1193 | - |
N-Acetyl-S-(3-(3-methylbut-2-enyl)-5-(4-phenyl)phenylpent-2Z-en-1-yl)-L-cysteine | competitive inhibition | Homo sapiens | |
0.3772 | - |
N-Acetyl-S-(3-(3-methylbut-2-enyl)-5-(4-phenyl)phenylpent-2Z-en-1-yl)-L-cysteine | uncompetitive inhibition | Homo sapiens | |
0.6144 | - |
N-Acetyl-S-(3-(3-methylbut-2-enyl)-5-(4-phenyl)phenylpent-2Z-en-1-yl)-L-cysteine | uncompetitive inhibition | Saccharomyces cerevisiae |