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Literature summary for 2.1.1.100 extracted from

  • Febo-Ayala, W.; Morera-Felix, S.L.; Hrycyna, C.A.; Thompson, D.H.
    Functional reconstitution of the integral membrane enzyme, isoprenylcysteine carboxyl methyltransferase, in synthetic bolalipid membrane vesicles (2006), Biochemistry, 45, 14683-14694.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.100

Cloned(Commentary)

Cloned (Comment) Organism
His10myc3-tagged Ste14p expressed in Saccharomyces cereVisiae Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Saccharomyces cerevisiae 16020
-
vesicle Ste14p activity is lost in vesicles composed of 75-100 mol% C20BAS and 0-100 mol% C32BAS but retained in vesicles with 0-50 mol% C20BAS and 0-100 mol% C32phytBAS Saccharomyces cerevisiae 31982
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by Talon metal affinity chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + N-acetyl-S-farnesyl-L-cysteine
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N-acetyl-S-farnesyl-L-cysteine methyl ester
-
 ?

Synonyms

Synonyms Comment Organism
isoprenylcysteine carboxyl methyltransferase
-
 Saccharomyces cerevisiae
Ste14p
-
 Saccharomyces cerevisiae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 44 for the Escherichia coli polar lipid (control), Ste14p activity remains essentially constant when the assay temperature is maintained at 10, 16, 32, or 44°C, activity in DMPC is unchanged below (16 °C) and above (32 °C) its phase transition temperature, yielding activity levels that are the same as the Escherichia coli polar lipid values within experimental error, for C20-BAS activity is only 5% of that observed for the Escherichia coli polar lipid above (32 °C) and below (10 °C) the phase transition temperature of C20BAS Saccharomyces cerevisiae