KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
selenate | pH 7.2, 30°C, holenzyme complex | Enterobacter cloacae | |
5.5 | - |
selenate | pH 7.2, 30°C, isolated alpha subunit | Enterobacter cloacae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Enterobacter cloacae | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | non-heme iron, 18 mol per mol of enzyme | Enterobacter cloacae | |
Molybdenum | 0.6 mol per mol of enzyme | Enterobacter cloacae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
3* 100, alpha-subunit, 3 * 55000, beta-subunit, 3 * 36000, gamma-subunit, SDS-PAGE | Enterobacter cloacae |
55000 | - |
3* 100, alpha-subunit, 3 * 55000, beta-subunit, 3 * 36000, gamma-subunit, SDS-PAGE | Enterobacter cloacae |
600000 | - |
gel filtration | Enterobacter cloacae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | - |
expression under aerobic conditions, poor expression during anaerobic growth on nitrate | - |
Enterobacter cloacae SLD1a-1 / ATCC 700258 | - |
expression under aerobic conditions, poor expression during anaerobic growth on nitrate | - |
Purification (Comment) | Organism |
---|---|
- |
Enterobacter cloacae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
500 | - |
pH 7.2, 30°C | Enterobacter cloacae |
Storage Stability | Organism |
---|---|
-20°C, enzyme extracted with Thesit retains 50% activity after being frozen for prolonged periods | Enterobacter cloacae |
4°C, enzyme extracted with Thesit remains active for 24 h | Enterobacter cloacae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no substrate: nitrate, sulfate, perchlorate, thiosulfate | Enterobacter cloacae | ? | - |
? | |
additional information | no substrate: nitrate, sulfate, perchlorate, thiosulfate | Enterobacter cloacae SLD1a-1 / ATCC 700258 | ? | - |
? | |
selenate + electron donor | - |
Enterobacter cloacae | selenite + H2O + oxidized donor | - |
? | |
selenate + electron donor | - |
Enterobacter cloacae SLD1a-1 / ATCC 700258 | selenite + H2O + oxidized donor | - |
? |
Subunits | Comment | Organism |
---|---|---|
nonamer | 3* 100, alpha-subunit, 3 * 55000, beta-subunit, 3 * 36000, gamma-subunit, SDS-PAGE | Enterobacter cloacae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | 0.9 mol per mol of enzyme | Enterobacter cloacae |