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Literature summary for 1.97.1.4 extracted from
- Biochemical and functional properties of a pyruvate formate-lyase (PFL)-activating system in Streptococcus mutans (2003), Oral Microbiol. Immunol., 18, 293-297.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | in dental plaque, under anaerobic conditions, enzyme is always kept active | Streptococcus mutans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel filtration | Streptococcus mutans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus mutans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptococcus mutans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:anaerobically-grown cell | - |
Streptococcus mutans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 105 | - |
pH 6.8, 30°C | Streptococcus mutans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, presence of Fe(SO4)2(NH4)2 and dithiothreitol, relatively stable | Streptococcus mutans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + NADH + formate acetyltransferase-glycine | - |
Streptococcus mutans | 5'-deoxyadenosine + methionine + NAD+ + formate acetyltransferase-glycine-2-yl-radical | 40% of activity with reduced methyl viologen | ? |  |
| S-adenosyl-L-methionine + NADPH + formate acetyltransferase-glycine | 95% of activity with reduced methyl viologen | Streptococcus mutans | 5'-deoxyadenosine + methionine + NADP+ + formate acetyltransferase-glycine-2-yl-radical | - |
? | |
| S-adenosyl-L-methionine + reduced methyl viologen + formate acetyltransferase-glycine | - |
Streptococcus mutans | 5'-deoxyadenosine + methionine + methyl viologen + formate acetyltransferase-glycine-2-yl-radical | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Streptococcus mutans |
| 7.7 | - |
20% of activity at pH 6.8 | Streptococcus mutans |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Streptococcus mutans | isoelectric focusing | - |
7.8 |
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Release 2023.1 (January 2023)
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