Literature summary for 1.97.1.12 extracted from

Shubin, V.; Terekhova, I.; Bolychevtseva, Y.; El-Mohsnawy, E.; Roegner, M.; Maentele, W.; Kopczak, M.; Dzafic, E.
Thermostability of photosystem I trimers and monomers from the cyanobacterium Thermosynechococcus elongatus (2017), Spectrochim. Acta A, 179, 17-22 .

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Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Thermosynechococcus vestitus	P0A405 AND P0A407 AND P0A415	genes psaA, psaB, and PsaC	-

Subunits

Subunits	Comment	Organism
monomer	inside the trimer, subunits PsaL, PsaI and PsaM (5 alpha-helices in total) are located in the center of the trimerization domain of PSI, thereby forming most of the contacts between the monomers	Thermosynechococcus vestitus
trimer	inside the trimer, subunits PsaL, PsaI and PsaM (5 alpha-helices in total) are located in the center of the trimerization domain of PSI, thereby forming most of the contacts between the monomers	Thermosynechococcus vestitus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
20	100	analysis of thermostability of oligomeric PSI complexes. The thermal profile of the spectral shift of alpha-helices bands confirms the same two temperature intervals for PSI monomers and only one interval for trimers. Spectra at 20-100°C show distinct changes in the Amide I region of PSI complexes as a function of the rising temperature. Absorbance at the Amide I maximum of PSI monomers, gradually drops in two temperature intervals, i.e. 60-75°C and 80-90°C. In contrast, absorbance at the Amide I maximum of PSI trimers drops only in one temperature interval 80-95°C. Spectral changes of PSI trimers and monomers heated up to 100°C are irreversible due to protein denaturation and non-specific aggregation of complexes leading to new absorption bands. Thermal denaturation profiles, detailed overview, The enzyme shows denaturation at the monomer-monomer-interface at over 60°C, and denaturation/aggregation at the trimer-lipid interface at over 80°C. Oligomerization is one strategy to increase thermostability of PSI complexes	Thermosynechococcus vestitus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Thermosynechococcus vestitus

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Release 2023.1 (January 2023)