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Literature summary for 1.97.1.12 extracted from

  • Farkas, D.; Franzen, L.G.; Hansson, Ö.
    Cloning, expression and purification of the luminal domain of spinach photosystem 1 subunit PsaF functional in binding to plastocyanin and with a disulfide bridge required for folding (2011), Protein Expr. Purif., 78, 156-166.
    View publication on PubMed
Search for more literature for 1.97.1.12

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the luminal domain of spinach photosystem 1 subunit PsaF, expressed in Escherichia coli BL21 (DE3) using a pET32 Xa/LIC thioredoxin fusion system Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
reduced plastocyanin + oxidized ferredoxin + hv Spinacia oleracea
-
 oxidized plastocyanin + reduced ferredoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of the luminal domain of spinach photosystem 1 subunit PsaF Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced plastocyanin + oxidized ferredoxin + hv
-
 Spinacia oleracea oxidized plastocyanin + reduced ferredoxin
-
 ?
reduced plastocyanin + oxidized ferredoxin + hv the photosystem 1 subunit PsaF is involved in the docking of the electron-donor proteins plastocyanin and cytochrome c6, the recombinant protein binds to plastocyanin by a specific, native-like, electrostatic interaction Spinacia oleracea oxidized plastocyanin + reduced ferredoxin
-
 ?

Subunits

Subunits Comment Organism
? 21000-22000 Da, photosystem 1 subunit PsaF that is involved in the docking of the electron-donor proteins plastocyanin and cytochrome c6, SDS-PAGE Spinacia oleracea

Synonyms

Synonyms Comment Organism
PsaF
-
 Spinacia oleracea