BRENDA - Enzyme Database
show all sequences of 1.9.98.1

Electron transport systems of the chemoautotroph Ferrobacillus ferrooxidans II. Purification and properties of a heat-labile iron-cytochrome c reductase

Yates, M.G.; Nason, A.; J. Biol. Chem. 241, 4872-4880 (1966)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-oxo-3-phenylpropanoate
100% inhibition at 0.08 mM
Acidithiobacillus ferrooxidans
Cr2+
slight inhibition
Acidithiobacillus ferrooxidans
Cu2+
100% inhibition at 0.1 mM
Acidithiobacillus ferrooxidans
cysteine
100% inhibition at 0.2 mM
Acidithiobacillus ferrooxidans
Fe3+
48% inhibition at 1 mM
Acidithiobacillus ferrooxidans
additional information
not inhibitory: atebrin, amytal, antimycin A
Acidithiobacillus ferrooxidans
Ni2+
slight inhibition
Acidithiobacillus ferrooxidans
p-hydroxymercuribenzoate
100% inhibition at 0.5 mM, inhibition can be reversed by reduced glutathione or cysteine
Acidithiobacillus ferrooxidans
reduced glutathione
67% inhibition at 1 mM
Acidithiobacillus ferrooxidans
Sodium sulfide
-
Acidithiobacillus ferrooxidans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0275
-
cytochrome c
-
Acidithiobacillus ferrooxidans
1.6
-
Fe2+
-
Acidithiobacillus ferrooxidans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe
hemoprotein
Acidithiobacillus ferrooxidans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ferrocytochrome c + Fe3+
Acidithiobacillus ferrooxidans
close association of enzyme with respiration chain
ferricytochrome c + Fe2+
-
Acidithiobacillus ferrooxidans
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acidithiobacillus ferrooxidans
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Acidithiobacillus ferrooxidans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.006
-
-
Acidithiobacillus ferrooxidans
Storage Stability
Storage Stability
Organism
0-5C, several weeks without loss of activity
Acidithiobacillus ferrooxidans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ferrocytochrome c + Fe3+
-
396332
Acidithiobacillus ferrooxidans
ferricytochrome c + Fe2+
-
396332
Acidithiobacillus ferrooxidans
?
ferrocytochrome c + Fe3+
close association of enzyme with respiration chain
396332
Acidithiobacillus ferrooxidans
ferricytochrome c + Fe2+
-
396332
Acidithiobacillus ferrooxidans
-
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
0.5
-
several weeks, no loss of activity
Acidithiobacillus ferrooxidans
22
-
8 h, no loss of activity
Acidithiobacillus ferrooxidans
40
-
20 min, 10% loss of activity
Acidithiobacillus ferrooxidans
60
-
5 min, 46% loss of activity
Acidithiobacillus ferrooxidans
90
-
1 min, 74% loss of activity
Acidithiobacillus ferrooxidans
100
-
1 min, complete loss of activity
Acidithiobacillus ferrooxidans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.7
6.2
-
Acidithiobacillus ferrooxidans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
6.7
pH 5: about 50% of maximum activity, pH 6.7: about 25% of maximum activity
Acidithiobacillus ferrooxidans
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome
most highly purified fraction of the enzyme contains cytochrome c and apparently nonfunctional cytochrome b
Acidithiobacillus ferrooxidans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome
most highly purified fraction of the enzyme contains cytochrome c and apparently nonfunctional cytochrome b
Acidithiobacillus ferrooxidans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-oxo-3-phenylpropanoate
100% inhibition at 0.08 mM
Acidithiobacillus ferrooxidans
Cr2+
slight inhibition
Acidithiobacillus ferrooxidans
Cu2+
100% inhibition at 0.1 mM
Acidithiobacillus ferrooxidans
cysteine
100% inhibition at 0.2 mM
Acidithiobacillus ferrooxidans
Fe3+
48% inhibition at 1 mM
Acidithiobacillus ferrooxidans
additional information
not inhibitory: atebrin, amytal, antimycin A
Acidithiobacillus ferrooxidans
Ni2+
slight inhibition
Acidithiobacillus ferrooxidans
p-hydroxymercuribenzoate
100% inhibition at 0.5 mM, inhibition can be reversed by reduced glutathione or cysteine
Acidithiobacillus ferrooxidans
reduced glutathione
67% inhibition at 1 mM
Acidithiobacillus ferrooxidans
Sodium sulfide
-
Acidithiobacillus ferrooxidans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0275
-
cytochrome c
-
Acidithiobacillus ferrooxidans
1.6
-
Fe2+
-
Acidithiobacillus ferrooxidans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe
hemoprotein
Acidithiobacillus ferrooxidans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ferrocytochrome c + Fe3+
Acidithiobacillus ferrooxidans
close association of enzyme with respiration chain
ferricytochrome c + Fe2+
-
Acidithiobacillus ferrooxidans
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Acidithiobacillus ferrooxidans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.006
-
-
Acidithiobacillus ferrooxidans
Storage Stability (protein specific)
Storage Stability
Organism
0-5C, several weeks without loss of activity
Acidithiobacillus ferrooxidans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ferrocytochrome c + Fe3+
-
396332
Acidithiobacillus ferrooxidans
ferricytochrome c + Fe2+
-
396332
Acidithiobacillus ferrooxidans
?
ferrocytochrome c + Fe3+
close association of enzyme with respiration chain
396332
Acidithiobacillus ferrooxidans
ferricytochrome c + Fe2+
-
396332
Acidithiobacillus ferrooxidans
-
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
0.5
-
several weeks, no loss of activity
Acidithiobacillus ferrooxidans
22
-
8 h, no loss of activity
Acidithiobacillus ferrooxidans
40
-
20 min, 10% loss of activity
Acidithiobacillus ferrooxidans
60
-
5 min, 46% loss of activity
Acidithiobacillus ferrooxidans
90
-
1 min, 74% loss of activity
Acidithiobacillus ferrooxidans
100
-
1 min, complete loss of activity
Acidithiobacillus ferrooxidans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.7
6.2
-
Acidithiobacillus ferrooxidans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
6.7
pH 5: about 50% of maximum activity, pH 6.7: about 25% of maximum activity
Acidithiobacillus ferrooxidans
Other publictions for EC 1.9.98.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
396332
Yates
Electron transport systems of ...
Acidithiobacillus ferrooxidans
J. Biol. Chem.
241
4872-4880
1966
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10
2
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1
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1
-
3
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1
-
-
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1
1
2
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-
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6
-
1
1
-
1
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-
-
-
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1
-
-
-
-
10
-
2
-
1
-
1
-
-
-
1
-
-
1
1
2
-
-
-
6
-
1
1
-
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-
-
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