Literature summary for 1.9.98.1 extracted from

Yates, M.G.; Nason, A.
Electron transport systems of the chemoautotroph Ferrobacillus ferrooxidans II. Purification and properties of a heat-labile iron-cytochrome c reductase (1966), J. Biol. Chem., 241, 4872-4880.

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Inhibitors

Inhibitors	Comment	Organism	Structure
2-oxo-3-phenylpropanoate	100% inhibition at 0.08 mM	Acidithiobacillus ferrooxidans
Cr2+	slight inhibition	Acidithiobacillus ferrooxidans
Cu2+	100% inhibition at 0.1 mM	Acidithiobacillus ferrooxidans
cysteine	100% inhibition at 0.2 mM	Acidithiobacillus ferrooxidans
Fe3+	48% inhibition at 1 mM	Acidithiobacillus ferrooxidans
additional information	not inhibitory: atebrin, amytal, antimycin A	Acidithiobacillus ferrooxidans
Ni2+	slight inhibition	Acidithiobacillus ferrooxidans
p-hydroxymercuribenzoate	100% inhibition at 0.5 mM, inhibition can be reversed by reduced glutathione or cysteine	Acidithiobacillus ferrooxidans
reduced glutathione	67% inhibition at 1 mM	Acidithiobacillus ferrooxidans
Sodium sulfide	-	Acidithiobacillus ferrooxidans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0275	-	ferrocytochrome c	-	Acidithiobacillus ferrooxidans
1.6	-	Fe2+	-	Acidithiobacillus ferrooxidans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe	hemoprotein	Acidithiobacillus ferrooxidans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ferrocytochrome c + Fe3+	Acidithiobacillus ferrooxidans	close association of enzyme with respiration chain	ferricytochrome c + Fe2+	-	?

Organism

Organism	UniProt	Comment	Textmining
Acidithiobacillus ferrooxidans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partial	Acidithiobacillus ferrooxidans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.006	-	-	Acidithiobacillus ferrooxidans

Storage Stability

Storage Stability	Organism
0-5°C, several weeks without loss of activity	Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ferrocytochrome c + Fe3+	-	Acidithiobacillus ferrooxidans	ferricytochrome c + Fe2+	-	?
ferrocytochrome c + Fe3+	close association of enzyme with respiration chain	Acidithiobacillus ferrooxidans	ferricytochrome c + Fe2+	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
0.5	-	several weeks, no loss of activity	Acidithiobacillus ferrooxidans
22	-	8 h, no loss of activity	Acidithiobacillus ferrooxidans
40	-	20 min, 10% loss of activity	Acidithiobacillus ferrooxidans
60	-	5 min, 46% loss of activity	Acidithiobacillus ferrooxidans
90	-	1 min, 74% loss of activity	Acidithiobacillus ferrooxidans
100	-	1 min, complete loss of activity	Acidithiobacillus ferrooxidans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.7	6.2	-	Acidithiobacillus ferrooxidans

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	6.7	pH 5: about 50% of maximum activity, pH 6.7: about 25% of maximum activity	Acidithiobacillus ferrooxidans

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome	most highly purified fraction of the enzyme contains cytochrome c and apparently nonfunctional cytochrome b	Acidithiobacillus ferrooxidans

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Release 2023.1 (January 2023)