BRENDA - Enzyme Database show
show all sequences of 1.9.6.1

The periplasmic nitrate reductase NapABC supports luminal growth of Salmonella enterica serovar typhimurium during colitis

Lopez, C.A.; Rivera-Chavez, F.; Byndloss, M.X.; Baeumler, A.J.; Infect. Immun. 83, 3470-3478 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cloning of napABC, quantitative PCR-based expression analysis
Salmonella enterica
Engineering
Amino acid exchange
Commentary
Organism
additional information
constructin of a napA mutant strain CAL277, and of several other nap gene mutants, overview
Salmonella enterica
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Salmonella enterica
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 ferrocytochrome + 2 H+ + nitrate
Salmonella enterica
-
2 ferricytochrome + nitrite
-
-
r
2 ferrocytochrome + 2 H+ + nitrate
Salmonella enterica SL1344 AND CAL128
-
2 ferricytochrome + nitrite
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salmonella enterica
-
serovar typhimurium
-
Salmonella enterica SL1344 AND CAL128
-
serovar typhimurium
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 ferrocytochrome + 2 H+ + nitrate
-
742645
Salmonella enterica
2 ferricytochrome + nitrite
-
-
-
r
2 ferrocytochrome + 2 H+ + nitrate
-
742645
Salmonella enterica SL1344 AND CAL128
2 ferricytochrome + nitrite
-
-
-
r
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome c
-
Salmonella enterica
Cloned(Commentary) (protein specific)
Commentary
Organism
cloning of napABC, quantitative PCR-based expression analysis
Salmonella enterica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome c
-
Salmonella enterica
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
constructin of a napA mutant strain CAL277, and of several other nap gene mutants, overview
Salmonella enterica
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Salmonella enterica
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 ferrocytochrome + 2 H+ + nitrate
Salmonella enterica
-
2 ferricytochrome + nitrite
-
-
r
2 ferrocytochrome + 2 H+ + nitrate
Salmonella enterica SL1344 AND CAL128
-
2 ferricytochrome + nitrite
-
-
r
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 ferrocytochrome + 2 H+ + nitrate
-
742645
Salmonella enterica
2 ferricytochrome + nitrite
-
-
-
r
2 ferrocytochrome + 2 H+ + nitrate
-
742645
Salmonella enterica SL1344 AND CAL128
2 ferricytochrome + nitrite
-
-
-
r
General Information
General Information
Commentary
Organism
malfunction
Salmonella enterica serovar Typhimurium strains with defects in either nitrate reductase A (narG mutant) or the regulator inducing its transcription in the presence of high concentrations of nitrate (narL mutant) exhibit growth comparable to that of wild-type Salmonella enterica serovar Typhimurium. In contrast, a strain lacking a functional periplasmic nitrate reductase (napA mutant) exhibits a marked growth defect in the lumen of the colon. Inactivation of narP, encoding a response regulator that activates napABC transcription in response to low nitrate concentrations, significantly reduces the growth of Salmonella enterica serovar Typhimurium in the murine host gut lumen
Salmonella enterica
additional information
the Salmonella enterica serovar Typhimurium genome contains three nitrate reductases, encoded by the narGHI, narZYV, and napABC genes
Salmonella enterica
physiological function
Salmonella enterica serovar Typhimurium uses the periplasmic nitrate reductase to support its growth on the low nitrate concentrations encountered in the gut, a strategy that may be shared with other enteric pathogens
Salmonella enterica
General Information (protein specific)
General Information
Commentary
Organism
malfunction
Salmonella enterica serovar Typhimurium strains with defects in either nitrate reductase A (narG mutant) or the regulator inducing its transcription in the presence of high concentrations of nitrate (narL mutant) exhibit growth comparable to that of wild-type Salmonella enterica serovar Typhimurium. In contrast, a strain lacking a functional periplasmic nitrate reductase (napA mutant) exhibits a marked growth defect in the lumen of the colon. Inactivation of narP, encoding a response regulator that activates napABC transcription in response to low nitrate concentrations, significantly reduces the growth of Salmonella enterica serovar Typhimurium in the murine host gut lumen
Salmonella enterica
additional information
the Salmonella enterica serovar Typhimurium genome contains three nitrate reductases, encoded by the narGHI, narZYV, and napABC genes
Salmonella enterica
physiological function
Salmonella enterica serovar Typhimurium uses the periplasmic nitrate reductase to support its growth on the low nitrate concentrations encountered in the gut, a strategy that may be shared with other enteric pathogens
Salmonella enterica
Other publictions for EC 1.9.6.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741478
Cerqueira
Periplasmic nitrate reductase ...
Desulfovibrio desulfuricans, Methylotenera mobilis, Methylotenera mobilis JLW8
Acc. Chem. Res.
48
2875-2884
2015
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5
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1
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1
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5
5
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742645
Lopez
The periplasmic nitrate reduc ...
Salmonella enterica, Salmonella enterica SL1344 AND CAL128
Infect. Immun.
83
3470-3478
2015
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2
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3
3
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741958
Jacques
Kinetics of substrate inhibit ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides DSM 158
Biochim. Biophys. Acta
1837
1801-1809
2014
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1
1
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1
1
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742380
Sanchez
The nitrate-sensing NasST sys ...
Bradyrhizobium japonicum, Bradyrhizobium japonicum JCM 10833
Environ. Microbiol.
16
3263-3274
2014
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1
-
1
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1
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2
3
3
2
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742486
Dow
Characterization of a peripla ...
Escherichia coli
FEBS J.
281
246-260
2014
1
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742319
Gonzalez
-
Periplasmic nitrate reductase ...
Anaeromyxobacter dehalogenans, Bradyrhizobium japonicum, Campylobacter jejuni subsp. jejuni, Campylobacter jejuni subsp. jejuni ATCC 700819, Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1, Desulfitobacterium hafniense, Desulfovibrio desulfuricans, Escherichia coli, Paracoccus denitrificans, Paracoccus pantotrophus, Paracoccus pantotrophus GB17, Pseudomonas sp., Pseudomonas sp. G-179, Rhodobacter sphaeroides, Shewanella gelidimarina, Shewanella oneidensis, Wolinella succinogenes
Coord. Chem. Rev.
257
315-331
2013
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14
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28
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14
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25
25
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742648
Cerqueira
The sulfur shift an activatio ...
Desulfovibrio desulfuricans
Inorg. Chem.
52
10766-10772
2013
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1
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1
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1
1
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712967
Simpson
The periplasmic nitrate reduct ...
Shewanella amazonensis, Shewanella amazonensis SB2B, Shewanella baltica, Shewanella baltica OS155, Shewanella baltica OS185, Shewanella baltica OS195, Shewanella baltica OS223, Shewanella denitrificans, Shewanella denitrificans OS217, Shewanella frigidimarina, Shewanella halifaxensis, Shewanella loihica, Shewanella loihica PV-4, Shewanella oneidensis, Shewanella oneidensis MR-1 / ATCC 700550, Shewanella pealeana, Shewanella piezotolerans, Shewanella piezotolerans WP3, Shewanella putrefaciens, Shewanella putrefaciens CN-32, Shewanella sediminis, Shewanella sp., Shewanella sp. ANA-3, Shewanella sp. MR-4, Shewanella sp. MR-7, Shewanella sp. W3-18-1, Shewanella woodyi
Microbiology
156
302-312
2010
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42
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27
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696917
Durvasula
Effect of periplasmic nitrate ...
Paracoccus pantotrophus
Biotechnol. Prog.
25
973-979
2009
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698636
Stewart
Catabolite repression control ...
Paracoccus pantotrophus
J. Bacteriol.
191
996-1005
2009
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1
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699015
Hofmann
Density functional theory stud ...
Desulfovibrio desulfuricans
J. Biol. Inorg. Chem.
14
1023-1035
2009
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699199
Cerqueira
The effect of the sixth sulfur ...
Desulfovibrio desulfuricans
J. Comput. Chem.
30
2466-2484
2009
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711653
Van Alst
Compensatory periplasmic nitra ...
Pseudomonas aeruginosa
Can. J. Microbiol.
55
1133-1144
2009
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Gates
Voltammetric characterization ...
Paracoccus pantotrophus
Biochem. J.
409
159-168
2008
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695337
Coelho
Heterodimeric nitrate reductas ...
Cupriavidus necator H16
Acta Crystallogr. Sect. F
63
516-519
2007
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674891
Jepson
Spectropotentiometric and stru ...
Escherichia coli
J. Biol. Chem.
282
6425-6437
2006
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675847
Nilavongse
The NapF protein of the Escher ...
Escherichia coli
Microbiology
152
3227-3237
2006
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654561
Pittman
Electron transport through nit ...
Campylobacter jejuni
Biochem. Soc. Trans.
33
190-192
2005
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696082
Brondijk
NapGH components of the peripl ...
Escherichia coli K-12
Biochem. J.
379
47-55
2004
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395185
Steward
Periplasmic nitrate reductase ...
Escherichia coli K-12
J. Bacteriol.
184
1314-1323
2002
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