BRENDA - Enzyme Database show
show all sequences of 1.9.6.1

Roles of NapF, NapG and NapH, subunits of the Escherichia coli periplasmic nitrate reductase, in ubiquinol oxidation

Brondijk, T.H.; Fiegen, D.; Richardson, D.J.; Cole, J.A.; Mol. Microbiol. 44, 245-255 (2002)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Escherichia coli K-12
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
nitrate + ferrocytochrome
Escherichia coli K-12
NapG and H, but not NapF, are essential for electron transfer from ubiquinol to NapAB. NapC is essential for electron transfer from both ubiquinol and menaquinol to NapAB. It is proposed that NapG and H form an energy conserving quinol dehydrogenase functioning as either components of a proton pump or in a Q cycle, as electrons are transferred from ubiquinol to the membrane-bound cytochrome NapC
nitrite + ferricytochrome + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli K-12
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
nitrate + ferrocytochrome
NapG and H, but not NapF, are essential for electron transfer from ubiquinol to NapAB. NapC is essential for electron transfer from both ubiquinol and menaquinol to NapAB. It is proposed that NapG and H form an energy conserving quinol dehydrogenase functioning as either components of a proton pump or in a Q cycle, as electrons are transferred from ubiquinol to the membrane-bound cytochrome NapC
700256
Escherichia coli K-12
nitrite + ferricytochrome + H2O
-
-
-
?
nitrate + ferrocytochrome
the membrane-bound cytochrome NapC is essential for electron transfer from both ubiquinol and menaquinol to NapAB
700256
Escherichia coli K-12
nitrite + ferricytochrome + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
the nap operon of Escherichia coli K-12, encoding a periplasmic nitrate reductase, encodes seven proteins. The catalytic complex in the periplasm, NapA–NapB receives electrons from the quinol pool via the membrane-bound cytochrome NapC. Like NapA, B and C, NapD, is also essential for Nap activity. None of the remaining three polypeptides, NapF, G and H, which are predicted to encode non-heme, iron-sulfur proteins, are essential for Nap activity
Escherichia coli K-12
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome
the membrane-bound cytochrome NapC is essential for electron transfer from both ubiquinol and menaquinol to NapAB
Escherichia coli K-12
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome
the membrane-bound cytochrome NapC is essential for electron transfer from both ubiquinol and menaquinol to NapAB
Escherichia coli K-12
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Escherichia coli K-12
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
nitrate + ferrocytochrome
Escherichia coli K-12
NapG and H, but not NapF, are essential for electron transfer from ubiquinol to NapAB. NapC is essential for electron transfer from both ubiquinol and menaquinol to NapAB. It is proposed that NapG and H form an energy conserving quinol dehydrogenase functioning as either components of a proton pump or in a Q cycle, as electrons are transferred from ubiquinol to the membrane-bound cytochrome NapC
nitrite + ferricytochrome + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
nitrate + ferrocytochrome
NapG and H, but not NapF, are essential for electron transfer from ubiquinol to NapAB. NapC is essential for electron transfer from both ubiquinol and menaquinol to NapAB. It is proposed that NapG and H form an energy conserving quinol dehydrogenase functioning as either components of a proton pump or in a Q cycle, as electrons are transferred from ubiquinol to the membrane-bound cytochrome NapC
700256
Escherichia coli K-12
nitrite + ferricytochrome + H2O
-
-
-
?
nitrate + ferrocytochrome
the membrane-bound cytochrome NapC is essential for electron transfer from both ubiquinol and menaquinol to NapAB
700256
Escherichia coli K-12
nitrite + ferricytochrome + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
the nap operon of Escherichia coli K-12, encoding a periplasmic nitrate reductase, encodes seven proteins. The catalytic complex in the periplasm, NapA–NapB receives electrons from the quinol pool via the membrane-bound cytochrome NapC. Like NapA, B and C, NapD, is also essential for Nap activity. None of the remaining three polypeptides, NapF, G and H, which are predicted to encode non-heme, iron-sulfur proteins, are essential for Nap activity
Escherichia coli K-12
Other publictions for EC 1.9.6.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741478
Cerqueira
Periplasmic nitrate reductase ...
Desulfovibrio desulfuricans, Methylotenera mobilis, Methylotenera mobilis JLW8
Acc. Chem. Res.
48
2875-2884
2015
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1
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5
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742645
Lopez
The periplasmic nitrate reduc ...
Salmonella enterica, Salmonella enterica SL1344 AND CAL128
Infect. Immun.
83
3470-3478
2015
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3
3
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741958
Jacques
Kinetics of substrate inhibit ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides DSM 158
Biochim. Biophys. Acta
1837
1801-1809
2014
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742380
Sanchez
The nitrate-sensing NasST sys ...
Bradyrhizobium japonicum, Bradyrhizobium japonicum JCM 10833
Environ. Microbiol.
16
3263-3274
2014
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742486
Dow
Characterization of a peripla ...
Escherichia coli
FEBS J.
281
246-260
2014
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742319
Gonzalez
-
Periplasmic nitrate reductase ...
Anaeromyxobacter dehalogenans, Bradyrhizobium japonicum, Campylobacter jejuni subsp. jejuni, Campylobacter jejuni subsp. jejuni ATCC 700819, Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1, Desulfitobacterium hafniense, Desulfovibrio desulfuricans, Escherichia coli, Paracoccus denitrificans, Paracoccus pantotrophus, Paracoccus pantotrophus GB17, Pseudomonas sp., Pseudomonas sp. G-179, Rhodobacter sphaeroides, Shewanella gelidimarina, Shewanella oneidensis, Wolinella succinogenes
Coord. Chem. Rev.
257
315-331
2013
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14
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28
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14
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742648
Cerqueira
The sulfur shift an activatio ...
Desulfovibrio desulfuricans
Inorg. Chem.
52
10766-10772
2013
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712967
Simpson
The periplasmic nitrate reduct ...
Shewanella amazonensis, Shewanella amazonensis SB2B, Shewanella baltica, Shewanella baltica OS155, Shewanella baltica OS185, Shewanella baltica OS195, Shewanella baltica OS223, Shewanella denitrificans, Shewanella denitrificans OS217, Shewanella frigidimarina, Shewanella halifaxensis, Shewanella loihica, Shewanella loihica PV-4, Shewanella oneidensis, Shewanella oneidensis MR-1 / ATCC 700550, Shewanella pealeana, Shewanella piezotolerans, Shewanella piezotolerans WP3, Shewanella putrefaciens, Shewanella putrefaciens CN-32, Shewanella sediminis, Shewanella sp., Shewanella sp. ANA-3, Shewanella sp. MR-4, Shewanella sp. MR-7, Shewanella sp. W3-18-1, Shewanella woodyi
Microbiology
156
302-312
2010
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696917
Durvasula
Effect of periplasmic nitrate ...
Paracoccus pantotrophus
Biotechnol. Prog.
25
973-979
2009
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698636
Stewart
Catabolite repression control ...
Paracoccus pantotrophus
J. Bacteriol.
191
996-1005
2009
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1
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699015
Hofmann
Density functional theory stud ...
Desulfovibrio desulfuricans
J. Biol. Inorg. Chem.
14
1023-1035
2009
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699199
Cerqueira
The effect of the sixth sulfur ...
Desulfovibrio desulfuricans
J. Comput. Chem.
30
2466-2484
2009
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711653
Van Alst
Compensatory periplasmic nitra ...
Pseudomonas aeruginosa
Can. J. Microbiol.
55
1133-1144
2009
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684990
Gates
Voltammetric characterization ...
Paracoccus pantotrophus
Biochem. J.
409
159-168
2008
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695337
Coelho
Heterodimeric nitrate reductas ...
Cupriavidus necator H16
Acta Crystallogr. Sect. F
63
516-519
2007
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674891
Jepson
Spectropotentiometric and stru ...
Escherichia coli
J. Biol. Chem.
282
6425-6437
2006
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675847
Nilavongse
The NapF protein of the Escher ...
Escherichia coli
Microbiology
152
3227-3237
2006
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654561
Pittman
Electron transport through nit ...
Campylobacter jejuni
Biochem. Soc. Trans.
33
190-192
2005
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696082
Brondijk
NapGH components of the peripl ...
Escherichia coli K-12
Biochem. J.
379
47-55
2004
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395185
Steward
Periplasmic nitrate reductase ...
Escherichia coli K-12
J. Bacteriol.
184
1314-1323
2002
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700256
Brondijk
Roles of NapF, NapG and NapH, ...
Escherichia coli K-12
Mol. Microbiol.
44
245-255
2002
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4
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697968
Thomas
The periplasmic nitrate reduct ...
Escherichia coli K-12
FEMS Microbiol. Lett.
174
167-171
1999
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698555
Wang
The napF and narG nitrate redu ...
Escherichia coli
J. Bacteriol.
181
5303-5308
1999
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696375
Berks
Enzymes and associated electro ...
Cupriavidus necator, Escherichia coli, Paracoccus denitrificans, Paracoccus pantotrophus
Biochim. Biophys. Acta
1232
97-173
1995
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-
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4
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-
697696
Berks
Purification and characterizat ...
Paracoccus pantotrophus
Eur. J. Biochem.
220
117-124
1994
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3
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3
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697965
Sears
-
The identification of a peripl ...
Paracoccus denitrificans
FEMS MIcrobiol. Lett.
113
107-112
1993
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1
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698544
Siddiqui
Structure and function of a pe ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
J. Bacteriol.
175
5867-5876
1993
-
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1
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1
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1
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5
2
-
43
-
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395176
Sadana
Nitrate reductase from Achromo ...
Aliivibrio fischeri
Arch. Biochem. Biophys.
67
16-34
1957
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4
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