Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type and mutant Strep II-tagged enzymes | Campylobacter jejuni |
Protein Variants | Comment | Organism |
---|---|---|
C138H | site-directed mutagenesis, spectroscopic and electrochemical mutant characterization | Campylobacter jejuni |
C138M | site-directed mutagenesis, spectroscopic and electrochemical mutant characterization | Campylobacter jejuni |
N254K | site-directed mutagenesis, spectroscopic and electrochemical mutant characterization | Campylobacter jejuni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | in the enzyme bound hemes | Campylobacter jejuni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tetrathionate + reduced electron acceptor | Campylobacter jejuni | - |
2 thiosulfate + oxidized electron acceptor | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme functions as a tetrathionate reductase and a thiosulfate oxidase, respectively | Campylobacter jejuni | ? | - |
- |
|
tetrathionate + reduced electron acceptor | - |
Campylobacter jejuni | 2 thiosulfate + oxidized electron acceptor | - |
r | |
tetrathionate + reduced electron acceptor | for CjTsdA the maximum rate of tetrathionate reduction is approximately twice that of thiosulfate oxidation | Campylobacter jejuni | 2 thiosulfate + oxidized electron acceptor | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 37103, mature protein with two c-hemes, without the signal peptide, and with an N-terminal Strep II-tag preceded by three amino acids and followed by four amino acids, sequence calculation | Campylobacter jejuni |
Synonyms | Comment | Organism |
---|---|---|
CjTsdA | - |
Campylobacter jejuni |
di-heme TsdA | - |
Campylobacter jejuni |
thiosulfate dehydrogenase | - |
Campylobacter jejuni |
TsdA | - |
Campylobacter jejuni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | two hemes, heme 1 and heme 2. Distal ligation of CjTsdA Heme 2 is provided by Asn254 and Met255 | Campylobacter jejuni |
General Information | Comment | Organism |
---|---|---|
physiological function | thiosulfate dehydrogenases (TsdAs) are bidirectional bacterial di-heme enzymes that catalyze the interconversion of tetrathionate and thiosulfate at measurable rates in both directions, cf. EC 1.8.2.2, thiosulfate dehydrogenase. The active site heme 1 in the enzyme has His/Cys ligation in the ferric and ferrous states. In Campylobacter jejuni, TsdA heme 2 has His/Met ligation. Enzyme CjTsdA allows use of tetrathionate as a terminal respiratory electron sink | Campylobacter jejuni |