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Literature summary for 1.8.99.B2 extracted from

  • Sharmin, S.; Yoshino, E.; Kanao, T.; Kamimura, K.
    Characterization of a novel thiosulfate dehydrogenase from a marine acidophilic sulfur-oxidizing bacterium, Acidithiobacillus thiooxidans strain SH (2016), Biosci. Biotechnol. Biochem., 80, 273-278 .
    View publication on PubMed

Application

Application Comment Organism
additional information the Acidithiobacillus thiooxidans strain SH enzyme is isolated to develop a bioleaching process for NaCl-containing sulfide minerals. Thiosulfate-metabolizing activity is most important for bioleaching, as the sulfur moiety of sulfide minerals is thought to be metabolized via thiosulfate as an intermediate Acidithiobacillus thiooxidans

Inhibitors

Inhibitors Comment Organism Structure
2-heptyl-4-hydroxy-quinoline N-oxide strong inhibition Acidithiobacillus thiooxidans
2-heptyl-4-hydroxyquinoline N-oxide 88% inhibition at 0.001 mM Acidithiobacillus thiooxidans
2-heptyl-4-hydroxyquinoline N-oxide strong inhibition Acidithiobacillus thiooxidans
N-ethylmaleimide 16% inhibition at 1 mM Acidithiobacillus thiooxidans
Na+ inhibitory at high concentration above 200 mM; inihibits at high concentrations Acidithiobacillus thiooxidans
Na2SO3 70% inhibition at 1 mM Acidithiobacillus thiooxidans
Na2SO4 54% inhibition at 200 mM Acidithiobacillus thiooxidans
sulfite
-
Acidithiobacillus thiooxidans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Acidithiobacillus thiooxidans 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cl- activates Acidithiobacillus thiooxidans
KCl moderately stimulating, at 200 mM Acidithiobacillus thiooxidans
Mg2+ activates Acidithiobacillus thiooxidans
MgCl2 moderately stimulating at 200 mM Acidithiobacillus thiooxidans
MgSO4 slightly stimulating, at 200 mM Acidithiobacillus thiooxidans
additional information no stimulation of the enzyme by Na2SO4 at at 100 mM Acidithiobacillus thiooxidans
NaCl activates, best at 200 mM Acidithiobacillus thiooxidans
NaCl stimulates, best at 200 mM Acidithiobacillus thiooxidans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
99000
-
gel filtration Acidithiobacillus thiooxidans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 thiosulfate + ubiquinone Acidithiobacillus thiooxidans
-
tetrathionate + ubiquinol
-
?
2 thiosulfate + ubiquinone Acidithiobacillus thiooxidans SH
-
tetrathionate + ubiquinol
-
?

Organism

Organism UniProt Comment Textmining
Acidithiobacillus thiooxidans
-
-
-
Acidithiobacillus thiooxidans SH
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 71.4fold from the membrane fraction of strain SH by solubilization with n-dodecyl-beta-D-maltopyranoside, ultracentrifugation, anion exchange chromatography, dialysis, ultrafiltration, and gel filtration Acidithiobacillus thiooxidans
native enzyme 71.4fold from the solubilized membrane fraction by ultracentrifugation, anion exchange chromatography, dialysis, and ultrafiltration Acidithiobacillus thiooxidans

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:sulfur-grown cell NaCl at an optimum concentration of 0.35 M stimulates the growth of strain SH on elemental sulfur, while LiCl and KCl cannot sustain the growth of strain SH Acidithiobacillus thiooxidans
-
additional information NaCl at an optimum concentration of 0.35 M stimulated the growth of strain SH on elemental sulfur, while LiCl and KCl cannot sustain the growth Acidithiobacillus thiooxidans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
45
-
purified native enzyme, pH 4.0, 40°C Acidithiobacillus thiooxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 thiosulfate + ferricyanide
-
Acidithiobacillus thiooxidans tetrathionate + ferrocyanide
-
?
2 thiosulfate + ferricyanide artificial electron acceptor Acidithiobacillus thiooxidans tetrathionate + ferrocyanide
-
?
2 thiosulfate + ferricyanide
-
Acidithiobacillus thiooxidans SH tetrathionate + ferrocyanide
-
?
2 thiosulfate + ferricyanide artificial electron acceptor Acidithiobacillus thiooxidans SH tetrathionate + ferrocyanide
-
?
2 thiosulfate + ubiquinone
-
Acidithiobacillus thiooxidans tetrathionate + ubiquinol
-
?
2 thiosulfate + ubiquinone
-
Acidithiobacillus thiooxidans SH tetrathionate + ubiquinol
-
?
additional information no activity with cytochrome c Acidithiobacillus thiooxidans ?
-
?
additional information tetrathionate is the product of the TSD-oxidized thiosulfate, and ferricyanide or ubiquinone are the electron acceptors Acidithiobacillus thiooxidans ?
-
?
additional information no activity with cytochrome c Acidithiobacillus thiooxidans SH ?
-
?
additional information tetrathionate is the product of the TSD-oxidized thiosulfate, and ferricyanide or ubiquinone are the electron acceptors Acidithiobacillus thiooxidans SH ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 44000, SDS-PAGE Acidithiobacillus thiooxidans

Synonyms

Synonyms Comment Organism
tetrathionate-forming TSD
-
Acidithiobacillus thiooxidans
thiosulfate dehydrogenase
-
Acidithiobacillus thiooxidans
thiosulfate:quinone reductase
-
Acidithiobacillus thiooxidans
TQO
-
Acidithiobacillus thiooxidans
TSD
-
Acidithiobacillus thiooxidans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Acidithiobacillus thiooxidans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
inactivation Acidithiobacillus thiooxidans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Acidithiobacillus thiooxidans

pH Range

pH Minimum pH Maximum Comment Organism
2.5 5.5 activity range, profile overview Acidithiobacillus thiooxidans

Cofactor

Cofactor Comment Organism Structure
ferricyanide
-
Acidithiobacillus thiooxidans
additional information no activity with cytochrome c from horse heart Acidithiobacillus thiooxidans
additional information the enzyme contains no c-type heme molecule Acidithiobacillus thiooxidans
ubiquinone
-
Acidithiobacillus thiooxidans

General Information

General Information Comment Organism
evolution strain SH might have acquired a unique gene encoding the 44 kDa protein by horizontal transfer, as the protein with a high score and coverage is not found in other Acidithiobacillus thiooxidans genomes Acidithiobacillus thiooxidans
physiological function a thiosulfate metabolizing enzyme is required for the sulfur-grown cells to metabolize the resulting thiosulfate Acidithiobacillus thiooxidans