BRENDA - Enzyme Database show
show all sequences of 1.8.99.B2

Characterization of a novel thiosulfate dehydrogenase from a marine acidophilic sulfur-oxidizing bacterium, Acidithiobacillus thiooxidans strain SH

Sharmin, S.; Yoshino, E.; Kanao, T.; Kamimura, K.; Biosci. Biotechnol. Biochem. 80, 273-278 (2016)

Data extracted from this reference:

Application
Application
Commentary
Organism
additional information
the Acidithiobacillus thiooxidans strain SH enzyme is isolated to develop a bioleaching process for NaCl-containing sulfide minerals. Thiosulfate-metabolizing activity is most important for bioleaching, as the sulfur moiety of sulfide minerals is thought to be metabolized via thiosulfate as an intermediate
Acidithiobacillus thiooxidans
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-heptyl-4-hydroxy-quinoline N-oxide
strong inhibition
Acidithiobacillus thiooxidans
2-heptyl-4-hydroxyquinoline N-oxide
88% inhibition at 0.001 mM
Acidithiobacillus thiooxidans
2-heptyl-4-hydroxyquinoline N-oxide
strong inhibition
Acidithiobacillus thiooxidans
N-ethylmaleimide
; 16% inhibition at 1 mM
Acidithiobacillus thiooxidans
Na+
inhibitory at high concentration above 200 mM; inihibits at high concentrations
Acidithiobacillus thiooxidans
Na2SO3
70% inhibition at 1 mM
Acidithiobacillus thiooxidans
Na2SO4
54% inhibition at 200 mM
Acidithiobacillus thiooxidans
sulfite
-
Acidithiobacillus thiooxidans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Acidithiobacillus thiooxidans
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cl-
activates
Acidithiobacillus thiooxidans
KCl
moderately stimulating, at 200 mM
Acidithiobacillus thiooxidans
Mg2+
activates
Acidithiobacillus thiooxidans
MgCl2
moderately stimulating at 200 mM
Acidithiobacillus thiooxidans
MgSO4
slightly stimulating, at 200 mM
Acidithiobacillus thiooxidans
additional information
no stimulation of the enzyme by Na2SO4 at at 100 mM
Acidithiobacillus thiooxidans
NaCl
activates, best at 200 mM; stimulates, best at 200 mM
Acidithiobacillus thiooxidans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
99000
-
gel filtration
Acidithiobacillus thiooxidans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 thiosulfate + ubiquinone
Acidithiobacillus thiooxidans
-
tetrathionate + ubiquinol
-
-
?
2 thiosulfate + ubiquinone
Acidithiobacillus thiooxidans SH
-
tetrathionate + ubiquinol
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acidithiobacillus thiooxidans
-
-
-
Acidithiobacillus thiooxidans SH
-
-
-
Purification (Commentary)
Commentary
Organism
native enzyme 71.4fold from the membrane fraction of strain SH by solubilization with n-dodecyl-beta-D-maltopyranoside, ultracentrifugation, anion exchange chromatography, dialysis, ultrafiltration, and gel filtration; native enzyme 71.4fold from the solubilized membrane fraction by ultracentrifugation, anion exchange chromatography, dialysis, and ultrafiltration
Acidithiobacillus thiooxidans
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:sulfur-grown cell
NaCl at an optimum concentration of 0.35 M stimulates the growth of strain SH on elemental sulfur, while LiCl and KCl cannot sustain the growth of strain SH
Acidithiobacillus thiooxidans
-
additional information
NaCl at an optimum concentration of 0.35 M stimulated the growth of strain SH on elemental sulfur, while LiCl and KCl cannot sustain the growth
Acidithiobacillus thiooxidans
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
45
-
purified native enzyme, pH 4.0, 40C
Acidithiobacillus thiooxidans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 thiosulfate + ferricyanide
-
742121
Acidithiobacillus thiooxidans
tetrathionate + ferrocyanide
-
-
-
?
2 thiosulfate + ferricyanide
artificial electron acceptor
742121
Acidithiobacillus thiooxidans
tetrathionate + ferrocyanide
-
-
-
?
2 thiosulfate + ferricyanide
-
742121
Acidithiobacillus thiooxidans SH
tetrathionate + ferrocyanide
-
-
-
?
2 thiosulfate + ferricyanide
artificial electron acceptor
742121
Acidithiobacillus thiooxidans SH
tetrathionate + ferrocyanide
-
-
-
?
2 thiosulfate + ubiquinone
-
742121
Acidithiobacillus thiooxidans
tetrathionate + ubiquinol
-
-
-
?
2 thiosulfate + ubiquinone
-
742121
Acidithiobacillus thiooxidans SH
tetrathionate + ubiquinol
-
-
-
?
additional information
no activity with cytochrome c
742121
Acidithiobacillus thiooxidans
?
-
-
-
-
additional information
tetrathionate is the product of the TSD-oxidized thiosulfate, and ferricyanide or ubiquinone are the electron acceptors
742121
Acidithiobacillus thiooxidans
?
-
-
-
-
additional information
no activity with cytochrome c
742121
Acidithiobacillus thiooxidans SH
?
-
-
-
-
additional information
tetrathionate is the product of the TSD-oxidized thiosulfate, and ferricyanide or ubiquinone are the electron acceptors
742121
Acidithiobacillus thiooxidans SH
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 44000, SDS-PAGE
Acidithiobacillus thiooxidans
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
-
Acidithiobacillus thiooxidans
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
100
-
inactivation
Acidithiobacillus thiooxidans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
-
-
Acidithiobacillus thiooxidans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
2.5
5.5
activity range, profile overview
Acidithiobacillus thiooxidans
Cofactor
Cofactor
Commentary
Organism
Structure
ferricyanide
-
Acidithiobacillus thiooxidans
additional information
no activity with cytochrome c from horse heart; the enzyme contains no c-type heme molecule
Acidithiobacillus thiooxidans
ubiquinone
-
Acidithiobacillus thiooxidans
Application (protein specific)
Application
Commentary
Organism
additional information
the Acidithiobacillus thiooxidans strain SH enzyme is isolated to develop a bioleaching process for NaCl-containing sulfide minerals. Thiosulfate-metabolizing activity is most important for bioleaching, as the sulfur moiety of sulfide minerals is thought to be metabolized via thiosulfate as an intermediate
Acidithiobacillus thiooxidans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ferricyanide
-
Acidithiobacillus thiooxidans
additional information
no activity with cytochrome c from horse heart; the enzyme contains no c-type heme molecule
Acidithiobacillus thiooxidans
ubiquinone
-
Acidithiobacillus thiooxidans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-heptyl-4-hydroxy-quinoline N-oxide
strong inhibition
Acidithiobacillus thiooxidans
2-heptyl-4-hydroxyquinoline N-oxide
88% inhibition at 0.001 mM
Acidithiobacillus thiooxidans
2-heptyl-4-hydroxyquinoline N-oxide
strong inhibition
Acidithiobacillus thiooxidans
N-ethylmaleimide
; 16% inhibition at 1 mM
Acidithiobacillus thiooxidans
Na+
inhibitory at high concentration above 200 mM; inihibits at high concentrations
Acidithiobacillus thiooxidans
Na2SO3
70% inhibition at 1 mM
Acidithiobacillus thiooxidans
Na2SO4
54% inhibition at 200 mM
Acidithiobacillus thiooxidans
sulfite
-
Acidithiobacillus thiooxidans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Acidithiobacillus thiooxidans
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cl-
activates
Acidithiobacillus thiooxidans
KCl
moderately stimulating, at 200 mM
Acidithiobacillus thiooxidans
Mg2+
activates
Acidithiobacillus thiooxidans
MgCl2
moderately stimulating at 200 mM
Acidithiobacillus thiooxidans
MgSO4
slightly stimulating, at 200 mM
Acidithiobacillus thiooxidans
additional information
no stimulation of the enzyme by Na2SO4 at at 100 mM
Acidithiobacillus thiooxidans
NaCl
activates, best at 200 mM; stimulates, best at 200 mM
Acidithiobacillus thiooxidans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
99000
-
gel filtration
Acidithiobacillus thiooxidans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 thiosulfate + ubiquinone
Acidithiobacillus thiooxidans
-
tetrathionate + ubiquinol
-
-
?
2 thiosulfate + ubiquinone
Acidithiobacillus thiooxidans SH
-
tetrathionate + ubiquinol
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme 71.4fold from the membrane fraction of strain SH by solubilization with n-dodecyl-beta-D-maltopyranoside, ultracentrifugation, anion exchange chromatography, dialysis, ultrafiltration, and gel filtration; native enzyme 71.4fold from the solubilized membrane fraction by ultracentrifugation, anion exchange chromatography, dialysis, and ultrafiltration
Acidithiobacillus thiooxidans
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:sulfur-grown cell
NaCl at an optimum concentration of 0.35 M stimulates the growth of strain SH on elemental sulfur, while LiCl and KCl cannot sustain the growth of strain SH
Acidithiobacillus thiooxidans
-
additional information
NaCl at an optimum concentration of 0.35 M stimulated the growth of strain SH on elemental sulfur, while LiCl and KCl cannot sustain the growth
Acidithiobacillus thiooxidans
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
45
-
purified native enzyme, pH 4.0, 40C
Acidithiobacillus thiooxidans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 thiosulfate + ferricyanide
-
742121
Acidithiobacillus thiooxidans
tetrathionate + ferrocyanide
-
-
-
?
2 thiosulfate + ferricyanide
artificial electron acceptor
742121
Acidithiobacillus thiooxidans
tetrathionate + ferrocyanide
-
-
-
?
2 thiosulfate + ferricyanide
-
742121
Acidithiobacillus thiooxidans SH
tetrathionate + ferrocyanide
-
-
-
?
2 thiosulfate + ferricyanide
artificial electron acceptor
742121
Acidithiobacillus thiooxidans SH
tetrathionate + ferrocyanide
-
-
-
?
2 thiosulfate + ubiquinone
-
742121
Acidithiobacillus thiooxidans
tetrathionate + ubiquinol
-
-
-
?
2 thiosulfate + ubiquinone
-
742121
Acidithiobacillus thiooxidans SH
tetrathionate + ubiquinol
-
-
-
?
additional information
no activity with cytochrome c
742121
Acidithiobacillus thiooxidans
?
-
-
-
-
additional information
tetrathionate is the product of the TSD-oxidized thiosulfate, and ferricyanide or ubiquinone are the electron acceptors
742121
Acidithiobacillus thiooxidans
?
-
-
-
-
additional information
no activity with cytochrome c
742121
Acidithiobacillus thiooxidans SH
?
-
-
-
-
additional information
tetrathionate is the product of the TSD-oxidized thiosulfate, and ferricyanide or ubiquinone are the electron acceptors
742121
Acidithiobacillus thiooxidans SH
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 44000, SDS-PAGE
Acidithiobacillus thiooxidans
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
-
Acidithiobacillus thiooxidans
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
100
-
inactivation
Acidithiobacillus thiooxidans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
-
-
Acidithiobacillus thiooxidans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
2.5
5.5
activity range, profile overview
Acidithiobacillus thiooxidans
General Information
General Information
Commentary
Organism
evolution
strain SH might have acquired a unique gene encoding the 44 kDa protein by horizontal transfer, as the protein with a high score and coverage is not found in other Acidithiobacillus thiooxidans genomes
Acidithiobacillus thiooxidans
physiological function
a thiosulfate metabolizing enzyme is required for the sulfur-grown cells to metabolize the resulting thiosulfate
Acidithiobacillus thiooxidans
General Information (protein specific)
General Information
Commentary
Organism
evolution
strain SH might have acquired a unique gene encoding the 44 kDa protein by horizontal transfer, as the protein with a high score and coverage is not found in other Acidithiobacillus thiooxidans genomes
Acidithiobacillus thiooxidans
physiological function
a thiosulfate metabolizing enzyme is required for the sulfur-grown cells to metabolize the resulting thiosulfate
Acidithiobacillus thiooxidans
Other publictions for EC 1.8.99.B2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737877
Sharmin
Characterization of a novel th ...
Acidithiobacillus thiooxidans, Acidithiobacillus thiooxidans SH
Biosci. Biotechnol. Biochem.
80
273-278
2016
-
-
-
-
-
-
4
-
1
3
2
-
-
5
-
-
1
-
-
-
-
-
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
-
1
3
2
-
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
742121
Sharmin
Characterization of a novel t ...
Acidithiobacillus thiooxidans, Acidithiobacillus thiooxidans SH
Biosci. Biotechnol. Biochem.
80
273-278
2016
-
1
-
-
-
-
8
-
1
7
1
2
-
5
-
-
1
-
-
2
1
-
10
1
1
-
1
-
1
1
-
3
-
-
-
-
1
-
3
-
-
-
-
8
-
-
1
7
1
2
-
-
-
1
-
2
1
-
10
1
1
-
1
-
1
1
-
-
-
2
2
-
-
-
726008
Liu
Tetrathionate stimulated growt ...
Campylobacter jejuni
Mol. Microbiol.
88
173-188
2013
-
-
-
-
-
-
-
1
-
-
1
2
-
3
-
-
1
-
-
-
1
-
4
1
1
-
-
1
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
2
-
-
-
1
-
-
1
-
4
1
1
-
-
1
1
-
-
-
-
-
-
-
1
1
741675
Kikumoto
Tetrathionate-forming thiosul ...
Acidithiobacillus ferrooxidans
Appl. Environ. Microbiol.
79
113-120
2013
1
-
1
-
-
-
2
-
-
2
1
-
-
1
-
-
1
-
-
1
2
-
2
1
1
-
-
-
1
-
-
2
-
-
-
1
-
1
2
-
-
-
-
2
-
-
-
2
1
-
-
-
-
1
-
1
2
-
2
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
394129
Nakamura
Purification and properties of ...
Acidithiobacillus thiooxidans, Acidithiobacillus thiooxidans JCM 7814
Biosci. Biotechnol. Biochem.
65
102-108
2001
-
-
-
-
-
-
-
1
1
-
2
-
-
3
-
-
1
-
-
-
1
-
4
1
1
-
1
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
2
-
-
-
-
1
-
-
1
-
4
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-