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Literature summary for 1.8.99.B2 extracted from

  • Kikumoto, M.; Nogami, S.; Kanao, T.; Takada, J.; Kamimura, K.
    Tetrathionate-forming thiosulfate dehydrogenase from the acidophilic, chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans (2013), Appl. Environ. Microbiol., 79, 113-120 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.99.B2

Activating Compound

Activating Compound Comment Organism Structure
additional information cofactors might be required for the sulfate-dependent enzyme activation dissociated from the enzyme complex during purification Acidithiobacillus ferrooxidans

Cloned(Commentary)

Cloned (Comment) Organism
recombinant enzyme expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Acidithiobacillus ferrooxidans

Inhibitors

Inhibitors Comment Organism Structure
Na2SO4 150 mM Na-sulfate is required to generate maximum activity, 60% inhibition at 200 mM Acidithiobacillus ferrooxidans
sulfite activates 1.6fold at 2 mM, higher concentration of sulfite ions results in inhibition of the enzyme, and almost complete inhibition at 10 mM sulfite Acidithiobacillus ferrooxidans

Metals/Ions

Metals/Ions Comment Organism Structure
Na2SO4 150 mM Na-sulfate is required to generate maximum activity, 60% inhibition at 200 mM Acidithiobacillus ferrooxidans
sulfite activates 1.6fold at 2 mM, higher concentration of sulfite ions results in inhibition of the enzyme, and almost complete inhibition at 10 mM sulfite Acidithiobacillus ferrooxidans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 gel filtration Acidithiobacillus ferrooxidans

Organism

Organism UniProt Comment Textmining
Acidithiobacillus ferrooxidans B7J3E3
-
-

Purification (Commentary)

Purification (Comment) Organism
native periplasmic enzyme from strain ATCC 23270 and recombinant enzyme from Escherichia coli strain BL21(DE3) celll extract by ammonium sulfate fractionation, cation exchange chromatography, dialysis, hydrophobic interaction chromatography, ultrafilatration, and gel filtration Acidithiobacillus ferrooxidans

Source Tissue

Source Tissue Comment Organism Textmining
cell culture enzyme activity measured using ferricyanide as the electron acceptor is detected in cell extracts of Astrain ATCC 23270 grown on tetrathionate or sulfur, but no activity is detected in ferrous iron-grown cells Acidithiobacillus ferrooxidans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.5
-
 pH 2.5, 50°C, with ferricyanide Acidithiobacillus ferrooxidans
13.8
-
 pH 2.5, 70°C, with ferricyanide Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 thiosulfate + ferricyanide
-
 Acidithiobacillus ferrooxidans tetrathionate + ferrocyanide
-
 ?
additional information nonenzymatic reduction of ferricyanide is observed with thiosulfate Acidithiobacillus ferrooxidans ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 25000, SDS-PAGE Acidithiobacillus ferrooxidans

Synonyms

Synonyms Comment Organism
AFE_0042 locus name Acidithiobacillus ferrooxidans
tetrathionate-forming thiosulfate dehydrogenase
-
 Acidithiobacillus ferrooxidans
tetrathionate-forming TSD
-
 Acidithiobacillus ferrooxidans
TSD
-
 Acidithiobacillus ferrooxidans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Acidithiobacillus ferrooxidans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
-
 Acidithiobacillus ferrooxidans

Cofactor

Cofactor Comment Organism Structure
ferricyanide
-
 Acidithiobacillus ferrooxidans
additional information no heme is detected. Ubiquinone and cytochrome C are no cofactors Acidithiobacillus ferrooxidans

General Information

General Information Comment Organism
physiological function thiosulfate dehydrogenase plays a significant role in thiosulfate oxidation in the acidophilic, obligately chemolithoautotroph, Acidithiobacillus ferrooxidans Acidithiobacillus ferrooxidans