Any feedback?
Literature summary for 1.8.99.B2 extracted from
- Purification and properties of thiosulfate dehydrogenase from Acidithiobacillus thiooxidans JCM7814 (2001), Biosci. Biotechnol. Biochem., 65, 102-108.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.81 | - |
thiosulfate | - |
Acidithiobacillus thiooxidans |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Acidithiobacillus thiooxidans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
gel filtration | Acidithiobacillus thiooxidans |
| 27900 | - |
1 * 27900, SDS-PAGE | Acidithiobacillus thiooxidans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidithiobacillus thiooxidans | - |
JCM7814 | - |
| Acidithiobacillus thiooxidans JCM 7814 | - |
JCM7814 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using chromatography on Phenyl-Sepharose column, CM-cellulose column and hydroxylapatite column | Acidithiobacillus thiooxidans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.06 | - |
- |
Acidithiobacillus thiooxidans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiosulfate + oxidized acceptor | the activity does not couple with mammalian cytochrome c as electron acceptor | Acidithiobacillus thiooxidans | tetrathionate + reduced acceptor | - |
? | |
| thiosulfate + oxidized acceptor | ferricyanide acts as electron acceptor | Acidithiobacillus thiooxidans | tetrathionate + reduced acceptor | - |
? | |
| thiosulfate + oxidized acceptor | the activity does not couple with mammalian cytochrome c as electron acceptor | Acidithiobacillus thiooxidans JCM 7814 | tetrathionate + reduced acceptor | - |
? | |
| thiosulfate + oxidized acceptor | ferricyanide acts as electron acceptor | Acidithiobacillus thiooxidans JCM 7814 | tetrathionate + reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 27900, SDS-PAGE | Acidithiobacillus thiooxidans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Acidithiobacillus thiooxidans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable up to | Acidithiobacillus thiooxidans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Acidithiobacillus thiooxidans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | - |
Acidithiobacillus thiooxidans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme c | - |
Acidithiobacillus thiooxidans | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
