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Literature summary for 1.8.99.B1 extracted from
- Insights on a new PDI-like family: structural and functional analysis of a protein disulfide oxidoreductase from the bacterium Aquifex aeolicus (2005), J. Mol. Biol., 356, 155-164.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mutant enzymes C34S, C148S and C34S/C148S, are overexpressed in Escherichia coli | Aquifex aeolicus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure at 2.4 A resolution | Aquifex aeolicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C148S | mutant enzyme exhibits a lower activity compared to wild-type enzyme | Aquifex aeolicus |
| C34S | mutant enzyme retains nearly wild-type activity | Aquifex aeolicus |
| C34S/C148S | mutant enzyme shows no activity | Aquifex aeolicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26700 | - |
gel-filtration, electrospray mass spectroscopy | Aquifex aeolicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | O66753 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| wild-type enzyme and recombinant mutant enzymes C34S, C148S and C34S/C148S | Aquifex aeolicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme reveals an inherent glutathione-dependent thioltransferase activity | Aquifex aeolicus | ? | - |
? |  |
| [insulin]-disulfide + reduced dithiothreitol | - |
Aquifex aeolicus | [insulin]-dithiol + oxidized dithiothreitol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AaPDO | - |
Aquifex aeolicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Aquifex aeolicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Aquifex aeolicus |
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