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Literature summary for 1.8.99.B1 extracted from

  • Limauro, D.; Saviano, M.; Galdi, I.; Rossi, M.; Bartolucci, S.; Pedone, E.
    Sulfolobus solfataricus protein disulphide oxidoreductase: insight into the roles of its redox sites (2009), Protein Eng. Des. Sel., 22, 19-26.
    View publication on PubMed
Search for more literature for 1.8.99.B1

Cloned(Commentary)

Cloned (Comment) Organism
wild-type enzyme and mutant enzymes C155S/C158S, C41S/C44S and C173S/C178S are expressed in Escherichi coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
C155S/C158S with insulin as substrate the enzyme is completely inactive Saccharolobus solfataricus
C173S/C178S with insulin as substrate the enzyme shows lower activity than wild-type enzyme Saccharolobus solfataricus
C41S/C44S with insulin as substrate the enzyme shows lower activity than wild-type enzyme Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q980T5
-
-
Saccharolobus solfataricus P2 Q980T5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[insulin]-disulfide + reduced dithiothreitol
-
 Saccharolobus solfataricus [insulin]-dithiol + oxidized dithiothreitol
-
 ?
[insulin]-disulfide + reduced dithiothreitol
-
 Saccharolobus solfataricus P2 [insulin]-dithiol + oxidized dithiothreitol
-
 ?

Synonyms

Synonyms Comment Organism
protein disulfide oxidoreductase
-
 Saccharolobus solfataricus
SSO0192 locus name Saccharolobus solfataricus
SsPDO
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Saccharolobus solfataricus