Cloned (Comment) | Organism |
---|---|
wild-type enzyme and mutant enzymes C155S/C158S, C41S/C44S and C173S/C178S are expressed in Escherichi coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
C155S/C158S | with insulin as substrate the enzyme is completely inactive | Saccharolobus solfataricus |
C173S/C178S | with insulin as substrate the enzyme shows lower activity than wild-type enzyme | Saccharolobus solfataricus |
C41S/C44S | with insulin as substrate the enzyme shows lower activity than wild-type enzyme | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q980T5 | - |
- |
Saccharolobus solfataricus P2 | Q980T5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[insulin]-disulfide + reduced dithiothreitol | - |
Saccharolobus solfataricus | [insulin]-dithiol + oxidized dithiothreitol | - |
? | |
[insulin]-disulfide + reduced dithiothreitol | - |
Saccharolobus solfataricus P2 | [insulin]-dithiol + oxidized dithiothreitol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
protein disulfide oxidoreductase | - |
Saccharolobus solfataricus |
SSO0192 | locus name | Saccharolobus solfataricus |
SsPDO | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Saccharolobus solfataricus |