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Literature summary for 1.8.99.B1 extracted from
- Characterization of a multifunctional protein disulfide oxidoreductase from Sulfolobus solfataricus (2006), FEBS J., 273, 5407-5420.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26032 | - |
x * 26032, calculated from sequence | Saccharolobus solfataricus |
| 27100 | - |
gel filtration, electrospray mass spectrometry | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q980T5 | - |
- |
| Saccharolobus solfataricus P2 | Q980T5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [insulin]-disulfide + reduced dithiothreitol | - |
Saccharolobus solfataricus | [insulin]-dithiol + oxidized dithiothreitol | - |
? |  |
| [insulin]-disulfide + reduced dithiothreitol | - |
Saccharolobus solfataricus P2 | [insulin]-dithiol + oxidized dithiothreitol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 26032, calculated from sequence | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| protein disulfide oxidoreductase | - |
Saccharolobus solfataricus |
| SSO0192 | locus name | Saccharolobus solfataricus |
| SsPDO | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharolobus solfataricus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharolobus solfataricus | calculated from sequence | - |
4.7 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Saccharolobus solfataricus | when Sulfolobus solfataricus cells are incubated with H2O2, paraquat and tert-butyl hydroperoxide, the Sso0192 mRNA level increases. Specifically, a two-fold increase in transcriptional levels is observed within 30 min of the addition of tert-butyl hydroperoxide, while a slight induction is observed 30 min after paraquat and H2O2 treatment | up |
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