Literature summary for 1.8.99.2 extracted from

Basen, M.; Krueger, M.; Milucka, J.; Kuever, J.; Kahnt, J.; Grundmann, O.; Meyerdierks, A.; Widdel, F.; Shima, S.
Bacterial enzymes for dissimilatory sulfate reduction in a marine microbial mat (Black Sea) mediating anaerobic oxidation of methane (2011), Environ. Microbiol., 13, 1370-1379.

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General Stability

General Stability	Organism
total enzyme activity is significantly decreased by ammonium sulfate precipitation, leaving 20% of the initial activity in the supernatant	uncultured Desulfoarculaceae bacterium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20000	-	1 * 70000 + 1 * 20000, SDS-PAGE (in denaturing gels, only the small subunit is visible because the large AprA subunit probably overlapped with the highly abundant a-subunit of reverse methylcoenzyme M reductase)	uncultured Desulfoarculaceae bacterium
70000	-	1 * 70000 + 1 * 20000, SDS-PAGE (in denaturing gels, only the small subunit is visible because the large AprA subunit probably overlapped with the highly abundant a-subunit of reverse methylcoenzyme M reductase)	uncultured Desulfoarculaceae bacterium

Organism

Organism	UniProt	Comment	Textmining
uncultured Desulfoarculaceae bacterium	F6KEW4 and F6KEW3	large subunit AprA and small subunit AprB	-

Purification (Commentary)

Purification (Comment)	Organism
partial purification by ammonium sulfate precipitation and Q-Sepharose column chromatography	uncultured Desulfoarculaceae bacterium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
AMP + sulfite + [Fe(CN)6]3-	-	uncultured Desulfoarculaceae bacterium	adenylyl sulfate + [Fe(CN)6]4-	-	?

Subunits

Subunits	Comment	Organism
heterodimer	1 * 70000 + 1 * 20000, SDS-PAGE (in denaturing gels, only the small subunit is visible because the large AprA subunit probably overlapped with the highly abundant a-subunit of reverse methylcoenzyme M reductase)	uncultured Desulfoarculaceae bacterium

Synonyms

Synonyms	Comment	Organism
adenosine-5'-phosphosulfate reductase	-	uncultured Desulfoarculaceae bacterium
APR	-	uncultured Desulfoarculaceae bacterium
AprB	small subunit	uncultured Desulfoarculaceae bacterium
APS reductase	-	uncultured Desulfoarculaceae bacterium

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Release 2023.1 (January 2023)