Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
C249S | unreactive toward 4-vinylpyridine labelling | Mycobacterium tuberculosis |
C59S | unreactive toward 4-vinylpyridine labelling | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28513 | - |
1 * 28513, analytical ultracentrifugation | Mycobacterium tuberculosis |
28560 | - |
calculated from amino acid sequence | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap chelating column chromatography | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.6 | - |
untagged APS reductase using Escherichia coli thioredoxin | Mycobacterium tuberculosis |
5.1 | - |
His-tagged APS reductase using Escherichia coli thioredoxin | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenylyl sulfate + reduced thioredoxin | - |
Mycobacterium tuberculosis | AMP + sulfite + oxidized thioredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28513, analytical ultracentrifugation | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
adenosine-5'-phosphosulfate reductase | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Mycobacterium tuberculosis |