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Literature summary for 1.8.99.2 extracted from

  • Kim, S.K.; Rahman, A.; Bick, J.A.; Conover, R.C.; Johnson, M.K.; Mason, J.T.; Hirasawa, M.; Leustek, T.; Knaff, D.B.
    Properties of the cysteine residues and iron-sulfur cluster of the assimilatory 5'-adenylyl sulfate reductase from Pseudomonas aeruginosa (2004), Biochemistry, 43, 13478-13486.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli of a recombinant His-tagged protein and several mutants Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
C139S inactive mutant with no iron present Pseudomonas aeruginosa
C140S catalytic iron content is retained, but the mutant is inactive Pseudomonas aeruginosa
C228S inactive mutant with no iron present Pseudomonas aeruginosa
C231S inactive mutant with no iron present Pseudomonas aeruginosa
C256S catalytic iron content is retained, but the mutant is inactive Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Iron Cys139, Cys228 and Cys231 act as ligands to a [3Fe-4S] cluster Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
SDS-PAGE Pseudomonas aeruginosa
35430
-
mass spectrometry Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of a recombinant His-tagged protein and several mutants by Ni2+ affinity chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenylyl sulfate + reduced thioredoxin
-
Pseudomonas aeruginosa AMP + sulfite + oxidized thioredoxin
-
?

Subunits

Subunits Comment Organism
monomer 1 * 35000, SDS-PAGE, a homodimer can be formed in solution depending on the redox potential Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
APS reductase
-
Pseudomonas aeruginosa