Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli of a recombinant His-tagged protein and several mutants | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
C139S | inactive mutant with no iron present | Pseudomonas aeruginosa |
C140S | catalytic iron content is retained, but the mutant is inactive | Pseudomonas aeruginosa |
C228S | inactive mutant with no iron present | Pseudomonas aeruginosa |
C231S | inactive mutant with no iron present | Pseudomonas aeruginosa |
C256S | catalytic iron content is retained, but the mutant is inactive | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | Cys139, Cys228 and Cys231 act as ligands to a [3Fe-4S] cluster | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
SDS-PAGE | Pseudomonas aeruginosa |
35430 | - |
mass spectrometry | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of a recombinant His-tagged protein and several mutants by Ni2+ affinity chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenylyl sulfate + reduced thioredoxin | - |
Pseudomonas aeruginosa | AMP + sulfite + oxidized thioredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, SDS-PAGE, a homodimer can be formed in solution depending on the redox potential | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
APS reductase | - |
Pseudomonas aeruginosa |