Application | Comment | Organism |
---|---|---|
drug development | Srx may be a potential target for prevention or treatment of cancer, the colorimetric assay would be useful for high-throughput screening of Srx inhibitors as demonstrated | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
into the vector pET14b for expression in Escherichia coli BL21 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C99S | mutant, substitution of serine for Cys99 blocks the release of phosphate | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | screening for Srx inhibitors is carried out in an automated setup using 25000 chemicals | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + R-SH | Homo sapiens | - |
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-Srx proteins are purified from cell lysates by chromatography on a column on Ni-chelating Sepharose | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + R-SH | - |
Homo sapiens | peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Srx | - |
Homo sapiens |
sulfiredoxin | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
malachite green assay at room temperature | Homo sapiens |
30 | - |
kinetic assay | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
glutathione | - |
Homo sapiens | |
thioredoxin | - |
Homo sapiens |