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Literature summary for 1.8.98.1 extracted from

  • Costa, K.C.; Wong, P.M.; Wang, T.; Lie, T.J.; Dodsworth, J.A.; Swanson, I.; Burn, J.A.; Hackett, M.; Leigh, J.A.
    Protein complexing in a methanogen suggests electron bifurcation and electron delivery from formate to heterodisulfide reductase (2010), Proc. Natl. Acad. Sci. USA, 107, 11050-11055.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.98.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of HdrB1 and HdrB2 as C-terminally His6-tagged proteins in strain MM1263 and MM1264, respectively. The His tag does not affect enzyme activity or binding Methanococcus maripaludis

Protein Variants

Protein Variants Comment Organism
additional information construction of two mutant strains: MM1263 containing His-tagged HdrB1, MM1264 containing His-tagged HdrB2. The strain containing His-tagged HdrB1 and a null mutation in hdrB2 grows normally, as does the strain containing His-tagged HdrB2 and a null mutation in hdrB1. Because Hdr is essential, each His-tagged protein must be functional Methanococcus maripaludis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coenzyme B + coenzyme M + methanophenazine Methanococcus maripaludis
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 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-O3-phospho-L-threonine + dihydromethanophenazine
-
 ?
coenzyme B + coenzyme M + methanophenazine Methanococcus maripaludis MM1265
-
 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-O3-phospho-L-threonine + dihydromethanophenazine
-
 ?
additional information Methanococcus maripaludis Hdr complexes with F420-nonreducing hydrogenase, formate dehydrogenase, and formyl-MFR dehydrogenase, model of complex of proteins that interact with Hdr, overview ?
-
 ?
additional information Methanococcus maripaludis MM1265 Hdr complexes with F420-nonreducing hydrogenase, formate dehydrogenase, and formyl-MFR dehydrogenase, model of complex of proteins that interact with Hdr, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Methanococcus maripaludis
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-
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Methanococcus maripaludis MM1265
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged HdrB1 and HdrB2 by nickel affinity chromatography Methanococcus maripaludis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coenzyme B + coenzyme M + methanophenazine
-
 Methanococcus maripaludis N-(7-[(2-sulfoethyl)dithio]heptanoyl)-O3-phospho-L-threonine + dihydromethanophenazine
-
 ?
coenzyme B + coenzyme M + methanophenazine
-
 Methanococcus maripaludis MM1265 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-O3-phospho-L-threonine + dihydromethanophenazine
-
 ?
additional information Hdr complexes with F420-nonreducing hydrogenase, formate dehydrogenase, and formyl-MFR dehydrogenase, model of complex of proteins that interact with Hdr, overview Methanococcus maripaludis ?
-
 ?
additional information Hdr complexes with F420-nonreducing hydrogenase, formate dehydrogenase, and formyl-MFR dehydrogenase, model of complex of proteins that interact with Hdr, overview Methanococcus maripaludis MM1265 ?
-
 ?

Synonyms

Synonyms Comment Organism
HDR
-
 Methanococcus maripaludis
heterodisulfide reductase
-
 Methanococcus maripaludis

General Information

General Information Comment Organism
metabolism in methanogenic Archaea, the final step of methanogenesis generates methane and a heterodisulfide of coenzyme M and coenzyme B, CoM-S-S-CoB. Reduction of this heterodisulfide by heterodisulfide reductase regenerates HS-CoM and HS-CoB in an exergonic process, methanogenic pathway with hypothesized roles for the heterodisulfide reductase complex, overview. Hdr complexes with F420-nonreducing hydrogenase, formate dehydrogenase, and formyl-methanofuran dehydrogenase, model of complex of proteins that interact with Hdr, overview Methanococcus maripaludis
additional information a strain containing His-tagged HdrB1 and a null mutation in hdrB2 grows normally, as does a strain containing His-tagged HdrB2 and a null mutation in hdrB1. Because Hdr is essential, each His-tagged protein must be functional Methanococcus maripaludis