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Literature summary for 1.8.98.1 extracted from

  • Murakami, E.; Deppenmeier, U.; Ragsdale, S.W.
    Characterization of the intramolecular electron transfer pathway from 2-hydroxyphenazine to the heterodisulfide reductase from Methanosarcina thermophilo (2001), J. Biol. Chem., 276, 2432-2439.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Mersalyl acid
-
Methanosarcina thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.092
-
dihydromethanophenazine 25°C Methanosarcina thermophila
0.144
-
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine 25°C Methanosarcina thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains two [Fe4S4] clusters. Only the low spin heme and the high potential FeS cluster are involved in reduction of N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine. A 4 Fe cluster is the initial electron acceptor Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coenzyme B + coenzyme M + methanophenazine Methanosarcina thermophila
-
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine
-
?
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine Methanosarcina thermophila
-
coenzyme B + coenzyme M + methanophenazine
-
?

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
52
-
-
Methanosarcina thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coenzyme B + coenzyme M + methanophenazine
-
Methanosarcina thermophila N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine
-
?
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine
-
Methanosarcina thermophila coenzyme B + coenzyme M + methanophenazine
-
?
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine ping-pong mechanism Methanosarcina thermophila coenzyme B + coenzyme M + methanophenazine
-
?
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methyl viologen ping-pong mechanism Methanosarcina thermophila coenzyme B + coenzyme M + methyl viologen
-
?

Synonyms

Synonyms Comment Organism
HDR
-
Methanosarcina thermophila
heterodisulfide reductase
-
Methanosarcina thermophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
74
-
dihydromethanophenazine 25°C Methanosarcina thermophila

Cofactor

Cofactor Comment Organism Structure
heme contains two b-hemes. Only the low spin heme and the high potential FeS cluster are involved in reduction of N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine Methanosarcina thermophila