Crystallization (Comment) | Organism |
---|---|
structures of catalytic subunit YedY in both the molybdenum- and tungsten-substituted forms, five monomers per asymmetric unit | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
dimethyl sulfoxide | 25°C, pH not specified in the publication | Escherichia coli | |
22 | - |
Trimethylamine N-oxide | 25°C, pH not specified in the publication | Escherichia coli | |
27.9 | - |
tetrahydrothiophene oxide | 25°C, pH not specified in the publication | Escherichia coli | |
46.4 | - |
Trimethylamine N-oxide | presence of 1 M urea, 25°C, pH not specified in the publication | Escherichia coli | |
119 | - |
L-methionine sulfoxide | 25°C, pH not specified in the publication | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | the molybdenum ion is coordinated by three sulfur ligands, two contributed from the dithiolene sulfurs of the molybdopterin with a Mo-S distance of 2.4 A. The third sulfur ligand is Sgamma of residue C102 at a distance of 2.4 A | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P76342 and P76343 | P76342 i.e. catalytic subunit YedY/MsrP, P76343 i.e. heme-binding subunit YedZ/MsrQ | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethyl sulfoxide + reduced benzyl viologen | - |
Escherichia coli | dimethyl sulfide + oxidized benzyl viologen + H2O | - |
? | |
L-methionine sulfoxide + reduced benzyl viologen | - |
Escherichia coli | L-methionine + oxidized benzyl viologen + H2O | - |
? | |
tetrahydrothiophene oxide + reduced benzyl viologen | - |
Escherichia coli | tetrahydrothiophene + oxidized benzyl viologen + H2O | - |
? | |
trimethylamine N-oxide + reduced benzyl viologen | - |
Escherichia coli | trimethylamine + oxidized benzyl viologen + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | monomer in solution, light scattering analysis | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
YedY | - |
Escherichia coli |
YedZ | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.83 | - |
dimethyl sulfoxide | 25°C, pH not specified in the publication | Escherichia coli | |
14.8 | - |
Trimethylamine N-oxide | presence of 1 M urea, 25°C, pH not specified in the publication | Escherichia coli | |
18.1 | - |
tetrahydrothiophene oxide | 25°C, pH not specified in the publication | Escherichia coli | |
19 | - |
Trimethylamine N-oxide | 25°C, pH not specified in the publication | Escherichia coli | |
25.6 | - |
L-methionine sulfoxide | 25°C, pH not specified in the publication | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
molybdopterin | presence of one molybdenum molybdopterin not conjugated by an additional nucleotide. | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.215 | - |
L-methionine sulfoxide | 25°C, pH not specified in the publication | Escherichia coli | |
0.319 | - |
Trimethylamine N-oxide | presence of 1 M urea, 25°C, pH not specified in the publication | Escherichia coli | |
0.403 | - |
dimethyl sulfoxide | 25°C, pH not specified in the publication | Escherichia coli | |
0.649 | - |
tetrahydrothiophene oxide | 25°C, pH not specified in the publication | Escherichia coli | |
0.864 | - |
Trimethylamine N-oxide | 25°C, pH not specified in the publication | Escherichia coli |