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Literature summary for 1.8.5.9 extracted from

  • Inaba, K.; Murakami, S.; Nakagawa, A.; Iida, H.; Kinjo, M.; Ito, K.; Suzuki, M.
    Dynamic nature of disulphide bond formation catalysts revealed by crystal structures of DsbB (2009), EMBO J., 28, 779-791 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.5.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 15% (w/v) PEG3350, 0.1M magnesium formate and 0.1M MOPS (pH 7.0) Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[DsbA protein] with reduced L-cysteine residues + ubiquinone Escherichia coli overall reaction [DsbA protein] carrying a disulfide bond + ubiquinol
-
 ?
[DsbA protein] with reduced L-cysteine residues + [DsbB protein] carrying a disulfide bond Escherichia coli
-
 [DsbA protein] carrying a disulfide bond + [DsbB protein] with reduced L-cysteine residues
-
 ?
[DsbB protein] with reduced L-cysteine residues + ubiquinone Escherichia coli
-
 [DsbB protein] carrying a disulfide bond + ubiquinol
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A6M2
-
-

Purification (Commentary)

Purification (Comment) Organism
Superdex S200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[DsbA protein] with reduced L-cysteine residues + ubiquinone overall reaction Escherichia coli [DsbA protein] carrying a disulfide bond + ubiquinol
-
 ?
[DsbA protein] with reduced L-cysteine residues + [DsbB protein] carrying a disulfide bond
-
 Escherichia coli [DsbA protein] carrying a disulfide bond + [DsbB protein] with reduced L-cysteine residues
-
 ?
[DsbB protein] with reduced L-cysteine residues + ubiquinone
-
 Escherichia coli [DsbB protein] carrying a disulfide bond + ubiquinol
-
 ?

Synonyms

Synonyms Comment Organism
DsbB
-
 Escherichia coli

General Information

General Information Comment Organism
physiological function the enzyme oxidizes DsbA for continuous protein disulfide bond formation in the cell Escherichia coli