BRENDA - Enzyme Database
show all sequences of 1.8.5.8

Sulfidequinone oxidoreductase from echiuran worm Urechis unicinctus

Ma, Y.; Zhang, Z.; Shao, M.; Kang, K.; Tan, Z.; Li, J.; Mar. Biotechnol. 13, 93-107 (2011)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
EDTA
109.33% activity at 5 mM
Urechis unicinctus
glutathione
110.52% activity at 5 mM
Urechis unicinctus
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Urechis unicinctus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Zn2+
80.32% residual activity at 5 mM
Urechis unicinctus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0156
-
ubiquinone
at pH 8.5 and 37°C
Urechis unicinctus
0.043
-
Sulfide
with ubiquinone as cosubstrate, at pH 8.5 and 37°C
Urechis unicinctus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Urechis unicinctus
5739
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Urechis unicinctus
H2KXA2
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Urechis unicinctus
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography
Urechis unicinctus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
anal sac
-
Urechis unicinctus
-
body wall
-
Urechis unicinctus
-
coelomic fluid
-
Urechis unicinctus
-
epithelium
-
Urechis unicinctus
-
hindgut
-
Urechis unicinctus
-
midgut
highest expression
Urechis unicinctus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.12
-
at pH 8.0 and 25°C
Urechis unicinctus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sulfide + coenzyme Q2
-
745666
Urechis unicinctus
?
-
-
-
?
sulfide + ubiquinone
-
745666
Urechis unicinctus
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 50000, SDS-PAGE; x * 50500, calculated from amino acid sequence
Urechis unicinctus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Urechis unicinctus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
27
42
more than 60% activity between 27 and 42°C
Urechis unicinctus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Urechis unicinctus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8
9
more than 70% activity between pH 8.0 and 9.0
Urechis unicinctus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Urechis unicinctus
calculated from amino acid sequence
-
9
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
EDTA
109.33% activity at 5 mM
Urechis unicinctus
glutathione
110.52% activity at 5 mM
Urechis unicinctus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Urechis unicinctus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Zn2+
80.32% residual activity at 5 mM
Urechis unicinctus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0156
-
ubiquinone
at pH 8.5 and 37°C
Urechis unicinctus
0.043
-
Sulfide
with ubiquinone as cosubstrate, at pH 8.5 and 37°C
Urechis unicinctus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Urechis unicinctus
5739
-
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Urechis unicinctus
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Urechis unicinctus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
anal sac
-
Urechis unicinctus
-
body wall
-
Urechis unicinctus
-
coelomic fluid
-
Urechis unicinctus
-
epithelium
-
Urechis unicinctus
-
hindgut
-
Urechis unicinctus
-
midgut
highest expression
Urechis unicinctus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.12
-
at pH 8.0 and 25°C
Urechis unicinctus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sulfide + coenzyme Q2
-
745666
Urechis unicinctus
?
-
-
-
?
sulfide + ubiquinone
-
745666
Urechis unicinctus
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 50000, SDS-PAGE; x * 50500, calculated from amino acid sequence
Urechis unicinctus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Urechis unicinctus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
27
42
more than 60% activity between 27 and 42°C
Urechis unicinctus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Urechis unicinctus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8
9
more than 70% activity between pH 8.0 and 9.0
Urechis unicinctus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Urechis unicinctus
calculated from amino acid sequence
-
9
Other publictions for EC 1.8.5.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742905
Landry
H2S oxidation by nanodisc-emb ...
Homo sapiens
J. Biol. Chem.
292
11641-11649
2017
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-
1
-
-
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-
5
-
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1
-
1
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4
1
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6
-
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1
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1
1
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5
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1
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4
1
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6
-
-
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6
6
744373
Augustyn
Use of tissue metabolite anal ...
Homo sapiens
Biochemistry
56
986-996
2017
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-
-
-
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-
-
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1
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3
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3
-
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-
-
-
-
-
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-
743229
Jackson
Role of human sulfide quinone ...
Homo sapiens
Methods Enzymol.
554
255-270
2015
-
-
-
-
-
-
-
-
1
-
-
-
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1
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1
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1
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1
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-
745313
Libiad
Organization of the human mit ...
Homo sapiens
J. Biol. Chem.
289
30901-30910
2014
-
-
1
-
-
-
-
9
1
-
-
-
-
1
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1
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-
-
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6
1
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-
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5
-
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1
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-
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9
1
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1
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6
1
-
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5
-
-
-
-
-
-
-
-
9
9
744311
Jackson
-
Human sulfidequinone oxidored ...
Homo sapiens
Biochemistry
51
6804-6815
2012
-
-
1
-
-
-
-
-
1
-
-
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1
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1
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1
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1
1
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1
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1
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745667
Ma
Response of sulfide quinone o ...
Urechis unicinctus
Mar. Biotechnol.
14
245-251
2012
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1
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1
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1
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2
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2
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1
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1
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2
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2
-
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-
-
-
-
-
-
-
1
-
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1
-
-
745666
Ma
Sulfidequinone oxidoreductase ...
Urechis unicinctus
Mar. Biotechnol.
13
93-107
2011
2
-
1
-
-
-
1
2
1
-
-
-
-
1
-
1
1
-
-
6
1
-
2
1
1
1
-
-
1
1
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-
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1
-
2
-
1
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-
-
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1
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2
1
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1
1
-
6
1
-
2
1
1
1
-
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1
1
-
1
-
-
-
-
-
-
744869
Hildebrandt
Three enzymatic activities ca ...
Arenicola marina, Rattus norvegicus
FEBS J.
275
3352-3361
2008
-
-
-
-
-
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2
2
2
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2
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2
-
-
2
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1
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2
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2
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2
2
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2
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2
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1
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2
-
-
-
-
-
-
-
-
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-
-
-
-
745263
Vande Weghe
A fission yeast gene for mito ...
Schizosaccharomyces pombe, Schizosaccharomyces pombe JS23
J. Biol. Chem.
274
13250-13257
1999
-
-
1
-
-
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1
1
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2
-
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1
-
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2
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1
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1
1
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1
1
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1
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2
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